Development of simple sequence repeats (SSR) markers in Setaria Italica (poaceae) and cross-amplification in related species

Heng Sheng Lin; Chih Yun Chiang; Song Bin Chang; Chang Sheng Kuoh

doi:10.3390/ijms12117835

Development of simple sequence repeats (SSR) markers in Setaria Italica (poaceae) and cross-amplification in related species

Heng Sheng Lin, Chih Yun Chiang, Song Bin Chang, Chang Sheng Kuoh

Department of Life Sciences

Research output: Contribution to journal › Article › peer-review

33 Citations (Scopus)

Abstract

Foxtail millet is one of the world's oldest cultivated crops. It has been adopted as a model organism for providing a deeper understanding of plant biology. In this study, 45 simple sequence repeats (SSR) markers of Setaria italica were developed. These markers showing polymorphism were screened in 223 samples from 12 foxtail millet populations around Taiwan. The most common dinucleotide and trinucleotide repeat motifs are AC/TG (84.21%) and CAT (46.15%). The average number of alleles (Na), the average heterozygosities observed (Ho) and expected (He) are 3.73, 0.714, 0.587, respectively. In addition, 24 SSR markers had shown transferability to six related Poaceae species. These new markers provide tools for examining genetic relatedness among foxtail millet populations and other related species. It is suitable for germplasm management and protection in Poaceae.

Original language	English
Pages (from-to)	7835-7845
Number of pages	11
Journal	International journal of molecular sciences
Volume	12
Issue number	11
DOIs	https://doi.org/10.3390/ijms12117835
Publication status	Published - 2011 Nov

All Science Journal Classification (ASJC) codes

Catalysis
Molecular Biology
Spectroscopy
Computer Science Applications
Physical and Theoretical Chemistry
Organic Chemistry
Inorganic Chemistry

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10.3390/ijms12117835

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abstract = "Foxtail millet is one of the world's oldest cultivated crops. It has been adopted as a model organism for providing a deeper understanding of plant biology. In this study, 45 simple sequence repeats (SSR) markers of Setaria italica were developed. These markers showing polymorphism were screened in 223 samples from 12 foxtail millet populations around Taiwan. The most common dinucleotide and trinucleotide repeat motifs are AC/TG (84.21%) and CAT (46.15%). The average number of alleles (Na), the average heterozygosities observed (Ho) and expected (He) are 3.73, 0.714, 0.587, respectively. In addition, 24 SSR markers had shown transferability to six related Poaceae species. These new markers provide tools for examining genetic relatedness among foxtail millet populations and other related species. It is suitable for germplasm management and protection in Poaceae.",

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AU - Kuoh, Chang Sheng

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Development of simple sequence repeats (SSR) markers in Setaria Italica (poaceae) and cross-amplification in related species

Abstract

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