Diagnostic value of immunoglobulin g (igg) and igm anti-hepatitis e virus (hev) tests based on hev rna in an area where hepatitis e is not endemic

C. C. Lin, J. C. Wu, T. T. Chang, W. Y. Chang, M. L. Yu, A. W. Tam, S. C. Wang, Y. H. Huang, F. Y. Chang, S. D. Lee

Research output: Contribution to journalArticlepeer-review

71 Citations (Scopus)

Abstract

Acute hepatitis E (AHE) has rarely been reported in industrialized countries, but the rate of seroprevalence of hepatitis E virus (HEV) antibodies (anti-HEV) is inappropriately high. The sensitivity and specificity of the assay used to test for immunoglobulin G (IgG) and IgM anti-HEV have not been well established in areas where hepatitis E is not endemic (hereafter referred to as "nonendemic areas"). We collected serum samples from 13 AHE patients, 271 healthy subjects, and 160 other liver disease patients in Taiwan to test for HEV RNA by reverse transcription (RT)-PCR and for IgG and IgM anti-HEV by enzyme-linked immunosorbent assays. The sensitivities of IgG and IgM anti-HEV (relative to RT-PCR) were 86.7 and 53.3%, respectively. The specificities of IgG and IgM anti-HEV assays for diagnosing AHE were 92.1 and 98.6%, respectively. The rate of seroprevalence of IgG anti-HEV was 11% among healthy subjects in this nonendemic area, and it increased with age. In summary, IgG anti-HEV is a good diagnostic test for screening for AHE in nonendemic areas. The high rate of prevalence of anti-HEV in healthy subjects indicates that subclinical infection may exist.

Original languageEnglish
Pages (from-to)3915-3918
Number of pages4
JournalJournal of Clinical Microbiology
Volume38
Issue number11
DOIs
Publication statusPublished - 2000

All Science Journal Classification (ASJC) codes

  • Microbiology (medical)

Fingerprint Dive into the research topics of 'Diagnostic value of immunoglobulin g (igg) and igm anti-hepatitis e virus (hev) tests based on hev rna in an area where hepatitis e is not endemic'. Together they form a unique fingerprint.

Cite this