Dielectrophoresis and shear-enhanced sensitivity and selectivity of DNA hybridization for the rapid discrimination of Candida species

I. Fang Cheng, Huan Wen Han, Hsien-Chang Chang

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

We present a dielectrophoresis (DEP)-based microfluidic chip that is capable of enhancing the sensitivity and selectivity of DNA hybridization using an AC electric field and hydrodynamic shear in a continuous through-flow. Molecular DEP was employed to rapidly trap ssDNA molecules in a flowing solution to a cusp-shaped nanocolloid assembly on a microfluidic chip with a locally amplified AC electric field gradient. The detection time can be accelerated to sub-minute periods, and the sensitivity can reach the pico-molar level due to the AC DEP-enhanced molecule concentration (at an optimal AC frequency of 900kHz) in a small region (∼100μm 2) instead of the broad area used in a tank reactor (∼10 6μm 2). Continuous flow in a microchannel provides a constant and high shear rate that can shear off most non-specific target-probe binding to promote the discriminating selectivity. On-chip multi-target discrimination of Candida species can be achieved within a few minutes under optimal conditions.

Original languageEnglish
Pages (from-to)36-43
Number of pages8
JournalBiosensors and Bioelectronics
Volume33
Issue number1
DOIs
Publication statusPublished - 2012 Mar 15

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Microfluidics
Candida
Electrophoresis
DNA
Hydrodynamics
Electric fields
Molecules
Microchannels
Shear deformation

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

Cite this

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Dielectrophoresis and shear-enhanced sensitivity and selectivity of DNA hybridization for the rapid discrimination of Candida species. / Cheng, I. Fang; Han, Huan Wen; Chang, Hsien-Chang.

In: Biosensors and Bioelectronics, Vol. 33, No. 1, 15.03.2012, p. 36-43.

Research output: Contribution to journalArticle

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