Differential accumulation of heterochromatin as a cause for karyotype variation in Phalaenopsis orchids

Yen Yu Kao, Song-Bin Chang, Tsai Yun Lin, Chang Huain Hsieh, Yao Huang Chen, Wen Huei Chen, Chi Chang Chen

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Karyotypes of nine Phalaenopsis species and the closely related Doritis pulcherrima were compared based on Feulgenand DAPI-stained somatic metaphase chromosomes prepared from root tips. All species had the same chromosome number (2n = 2x = 38), but their karyotypes differed markedly in absolute chromosome size, relative chromosome size, and the position of the centromere. Both genome size and the amount of constitutive heterochromatin (CH) varied widely among the species studied, and there was a positive correlation between these two parameters. The distribution of CH in the genomes was non-random: one or both arms of long chromosomes usually possessed large blocks of CH, while the small chromosomes contained little or no CH. DAPI-staining revealed that most CH regions are rich in AT base pairs. We suggest that differential accumulation of CH is a major cause for karyotype variation in Phalaenopsis orchids.

Original languageEnglish
Pages (from-to)387-395
Number of pages9
JournalAnnals of Botany
Volume87
Issue number3
DOIs
Publication statusPublished - 2001 Jan 1

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Phalaenopsis
heterochromatin
Orchidaceae
karyotyping
chromosomes
genome
centromeres
metaphase
root tips
chromosome number

All Science Journal Classification (ASJC) codes

  • Plant Science

Cite this

Kao, Yen Yu ; Chang, Song-Bin ; Lin, Tsai Yun ; Hsieh, Chang Huain ; Chen, Yao Huang ; Chen, Wen Huei ; Chen, Chi Chang. / Differential accumulation of heterochromatin as a cause for karyotype variation in Phalaenopsis orchids. In: Annals of Botany. 2001 ; Vol. 87, No. 3. pp. 387-395.
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Differential accumulation of heterochromatin as a cause for karyotype variation in Phalaenopsis orchids. / Kao, Yen Yu; Chang, Song-Bin; Lin, Tsai Yun; Hsieh, Chang Huain; Chen, Yao Huang; Chen, Wen Huei; Chen, Chi Chang.

In: Annals of Botany, Vol. 87, No. 3, 01.01.2001, p. 387-395.

Research output: Contribution to journalArticle

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