Differential Ca2+ mobilization and mast cell degranulation by FcεRI- and GPCR-mediated signaling

Ying Chi Chen, Yu Chung Chang, Heng Ai Chang, Yu Shan Lin, Chiung Wen Tsao, Meng-Ru Shen, Wen-Tai Chiu

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Mast cells play a primary role in allergic diseases. During an allergic reaction, mast cell activation is initiated by cross-linking IgE-FcεRI complex by multivalent antigen resulting in degranulation. Additionally, G protein-coupled receptors also induce degranulation upon activation. However, the spatio-temporal relationship between Ca2+ mobilization and mast cell degranulation is not well understood. We investigated the relationship between oscillations in Ca2+ level and mast cell degranulation upon stimulation in rat RBL-2H3 cells. Nile red and Fluo-4 were used as probes for monitoring histamine and intracellular Ca2+ levels, respectively. Histamine release and Ca2+ oscillations in real-time were monitored using total internal reflection fluorescence microscopy (TIRFM). Mast cell degranulation followed immediately after FcεRI and GPCR-mediated Ca2+ increase. FcεRI-induced Ca2+ increase was higher and more sustained than that induced by GPCRs. However, no significant difference in mast cell degranulation rates was observed. Although intracellular Ca2+ release was both necessary and sufficient for mast cell degranulation, extracellular Ca2+ influx enhanced the process. Furthermore, cytosolic Ca2+ levels and mast cell degranulation were significantly decreased by downregulation of store-operated Ca2+ entry (SOCE) via Orai1 knockdown, 2-aminoethyl diphenylborinate (2-APB) or tubastatin A (TSA) treatment. Collectively, this study has demonstrated the role of Ca2+ signaling in regulating histamine degranulation.

Original languageEnglish
Pages (from-to)31-39
Number of pages9
JournalCell Calcium
Volume67
DOIs
Publication statusPublished - 2017 Nov 1

Fingerprint

Cell Degranulation
Mast Cells
Histamine
Histamine Release
G-Protein-Coupled Receptors
Fluorescence Microscopy
Immunoglobulin E
Hypersensitivity
Down-Regulation
Antigens

All Science Journal Classification (ASJC) codes

  • Physiology
  • Molecular Biology
  • Cell Biology

Cite this

Chen, Ying Chi ; Chang, Yu Chung ; Chang, Heng Ai ; Lin, Yu Shan ; Tsao, Chiung Wen ; Shen, Meng-Ru ; Chiu, Wen-Tai. / Differential Ca2+ mobilization and mast cell degranulation by FcεRI- and GPCR-mediated signaling. In: Cell Calcium. 2017 ; Vol. 67. pp. 31-39.
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abstract = "Mast cells play a primary role in allergic diseases. During an allergic reaction, mast cell activation is initiated by cross-linking IgE-FcεRI complex by multivalent antigen resulting in degranulation. Additionally, G protein-coupled receptors also induce degranulation upon activation. However, the spatio-temporal relationship between Ca2+ mobilization and mast cell degranulation is not well understood. We investigated the relationship between oscillations in Ca2+ level and mast cell degranulation upon stimulation in rat RBL-2H3 cells. Nile red and Fluo-4 were used as probes for monitoring histamine and intracellular Ca2+ levels, respectively. Histamine release and Ca2+ oscillations in real-time were monitored using total internal reflection fluorescence microscopy (TIRFM). Mast cell degranulation followed immediately after FcεRI and GPCR-mediated Ca2+ increase. FcεRI-induced Ca2+ increase was higher and more sustained than that induced by GPCRs. However, no significant difference in mast cell degranulation rates was observed. Although intracellular Ca2+ release was both necessary and sufficient for mast cell degranulation, extracellular Ca2+ influx enhanced the process. Furthermore, cytosolic Ca2+ levels and mast cell degranulation were significantly decreased by downregulation of store-operated Ca2+ entry (SOCE) via Orai1 knockdown, 2-aminoethyl diphenylborinate (2-APB) or tubastatin A (TSA) treatment. Collectively, this study has demonstrated the role of Ca2+ signaling in regulating histamine degranulation.",
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Differential Ca2+ mobilization and mast cell degranulation by FcεRI- and GPCR-mediated signaling. / Chen, Ying Chi; Chang, Yu Chung; Chang, Heng Ai; Lin, Yu Shan; Tsao, Chiung Wen; Shen, Meng-Ru; Chiu, Wen-Tai.

In: Cell Calcium, Vol. 67, 01.11.2017, p. 31-39.

Research output: Contribution to journalArticle

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T1 - Differential Ca2+ mobilization and mast cell degranulation by FcεRI- and GPCR-mediated signaling

AU - Chen, Ying Chi

AU - Chang, Yu Chung

AU - Chang, Heng Ai

AU - Lin, Yu Shan

AU - Tsao, Chiung Wen

AU - Shen, Meng-Ru

AU - Chiu, Wen-Tai

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