Differential effects of nitric oxide synthesis inhibitor on rat diaphragmatic microcirculation under basal conditions and after vasodilator stimulation

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Abstract

To assess the effect of endothelium-derived relaxing factor (EDRF) on the diaphragmatic microcirculation under basal conditions and after vasodilator stimulation, prepared diaphragms from anesthetized rats were studied. With bicarbonate-buffered Ringer's solution suffusing the diaphragm, the microcirculatory blood flow was recorded by laser-Doppler flowmetry (Q(LDF). Drugs were applied to the surface of the diaphragm. Five series of experiments were performed. In Series 1, the effects of acetylcholine (ACh, 10-4 M) and sodium nitroprusside (SNP, 3 x 10-6 M) were measured before and after 15 minutes suffusion of N(ω)-nitro-L-arginine methyl ester (L-NAME, 10-4 M). Trend analyses revealed that the vasodilator effect of SNP was not affected, while that of ACh was abolished by L-NAME. In Series 2, both ACh and SNP elicited a concentration-dependent increase in Q(LDF). In series 3, following 15 minutes suffusion with L-NAME (10-4 M), there was no change in baseline Q(LDF) and the ACh-induced Q(LDF) change was abolished, while the effect of SNP was maintained. In Series 4, five minutes of pretreatment with L-arginine (L-arg, 10-2 M), followed by co-administration of L-arg (10-2 M) and L-NAME (10-4 M) for another 15 minutes, did not prevent the inhibitory effect on ACh-induced Q(LDF) change by L-NAME. In Series 5, 20 minutes application of L-arg (10-2 M) alone did not affect baseline Q(LDF). Also, L-arg did not influence the ACh-induced vasodilating response, whereas it slightly increased the SNP-induced vasodilating response. These data suggest that the increase in endothelium-dependent blood flow stimulated by agonists may occur independently of low baseline EDRF activity in the diaphragmatic microvascular bed of anesthetized rats.

Original languageEnglish
Pages (from-to)788-796
Number of pages9
JournalJournal of the Formosan Medical Association
Volume93
Issue number9
Publication statusPublished - 1994

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Laser-Doppler Flowmetry
Microcirculation
Vasodilator Agents
NG-Nitroarginine Methyl Ester
Nitric Oxide
Single Nucleotide Polymorphism
Diaphragm
Endothelium-Dependent Relaxing Factors
Nitroprusside
Bicarbonates
Acetylcholine
Endothelium
Arginine
Pharmaceutical Preparations

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

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title = "Differential effects of nitric oxide synthesis inhibitor on rat diaphragmatic microcirculation under basal conditions and after vasodilator stimulation",
abstract = "To assess the effect of endothelium-derived relaxing factor (EDRF) on the diaphragmatic microcirculation under basal conditions and after vasodilator stimulation, prepared diaphragms from anesthetized rats were studied. With bicarbonate-buffered Ringer's solution suffusing the diaphragm, the microcirculatory blood flow was recorded by laser-Doppler flowmetry (Q(LDF). Drugs were applied to the surface of the diaphragm. Five series of experiments were performed. In Series 1, the effects of acetylcholine (ACh, 10-4 M) and sodium nitroprusside (SNP, 3 x 10-6 M) were measured before and after 15 minutes suffusion of N(ω)-nitro-L-arginine methyl ester (L-NAME, 10-4 M). Trend analyses revealed that the vasodilator effect of SNP was not affected, while that of ACh was abolished by L-NAME. In Series 2, both ACh and SNP elicited a concentration-dependent increase in Q(LDF). In series 3, following 15 minutes suffusion with L-NAME (10-4 M), there was no change in baseline Q(LDF) and the ACh-induced Q(LDF) change was abolished, while the effect of SNP was maintained. In Series 4, five minutes of pretreatment with L-arginine (L-arg, 10-2 M), followed by co-administration of L-arg (10-2 M) and L-NAME (10-4 M) for another 15 minutes, did not prevent the inhibitory effect on ACh-induced Q(LDF) change by L-NAME. In Series 5, 20 minutes application of L-arg (10-2 M) alone did not affect baseline Q(LDF). Also, L-arg did not influence the ACh-induced vasodilating response, whereas it slightly increased the SNP-induced vasodilating response. These data suggest that the increase in endothelium-dependent blood flow stimulated by agonists may occur independently of low baseline EDRF activity in the diaphragmatic microvascular bed of anesthetized rats.",
author = "Han-Yu Chang and Chang-Wen Chen and Tzuen-Ren Hsiue and Chen, {C. R.}",
year = "1994",
language = "English",
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pages = "788--796",
journal = "Journal of the Formosan Medical Association",
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T1 - Differential effects of nitric oxide synthesis inhibitor on rat diaphragmatic microcirculation under basal conditions and after vasodilator stimulation

AU - Chang, Han-Yu

AU - Chen, Chang-Wen

AU - Hsiue, Tzuen-Ren

AU - Chen, C. R.

PY - 1994

Y1 - 1994

N2 - To assess the effect of endothelium-derived relaxing factor (EDRF) on the diaphragmatic microcirculation under basal conditions and after vasodilator stimulation, prepared diaphragms from anesthetized rats were studied. With bicarbonate-buffered Ringer's solution suffusing the diaphragm, the microcirculatory blood flow was recorded by laser-Doppler flowmetry (Q(LDF). Drugs were applied to the surface of the diaphragm. Five series of experiments were performed. In Series 1, the effects of acetylcholine (ACh, 10-4 M) and sodium nitroprusside (SNP, 3 x 10-6 M) were measured before and after 15 minutes suffusion of N(ω)-nitro-L-arginine methyl ester (L-NAME, 10-4 M). Trend analyses revealed that the vasodilator effect of SNP was not affected, while that of ACh was abolished by L-NAME. In Series 2, both ACh and SNP elicited a concentration-dependent increase in Q(LDF). In series 3, following 15 minutes suffusion with L-NAME (10-4 M), there was no change in baseline Q(LDF) and the ACh-induced Q(LDF) change was abolished, while the effect of SNP was maintained. In Series 4, five minutes of pretreatment with L-arginine (L-arg, 10-2 M), followed by co-administration of L-arg (10-2 M) and L-NAME (10-4 M) for another 15 minutes, did not prevent the inhibitory effect on ACh-induced Q(LDF) change by L-NAME. In Series 5, 20 minutes application of L-arg (10-2 M) alone did not affect baseline Q(LDF). Also, L-arg did not influence the ACh-induced vasodilating response, whereas it slightly increased the SNP-induced vasodilating response. These data suggest that the increase in endothelium-dependent blood flow stimulated by agonists may occur independently of low baseline EDRF activity in the diaphragmatic microvascular bed of anesthetized rats.

AB - To assess the effect of endothelium-derived relaxing factor (EDRF) on the diaphragmatic microcirculation under basal conditions and after vasodilator stimulation, prepared diaphragms from anesthetized rats were studied. With bicarbonate-buffered Ringer's solution suffusing the diaphragm, the microcirculatory blood flow was recorded by laser-Doppler flowmetry (Q(LDF). Drugs were applied to the surface of the diaphragm. Five series of experiments were performed. In Series 1, the effects of acetylcholine (ACh, 10-4 M) and sodium nitroprusside (SNP, 3 x 10-6 M) were measured before and after 15 minutes suffusion of N(ω)-nitro-L-arginine methyl ester (L-NAME, 10-4 M). Trend analyses revealed that the vasodilator effect of SNP was not affected, while that of ACh was abolished by L-NAME. In Series 2, both ACh and SNP elicited a concentration-dependent increase in Q(LDF). In series 3, following 15 minutes suffusion with L-NAME (10-4 M), there was no change in baseline Q(LDF) and the ACh-induced Q(LDF) change was abolished, while the effect of SNP was maintained. In Series 4, five minutes of pretreatment with L-arginine (L-arg, 10-2 M), followed by co-administration of L-arg (10-2 M) and L-NAME (10-4 M) for another 15 minutes, did not prevent the inhibitory effect on ACh-induced Q(LDF) change by L-NAME. In Series 5, 20 minutes application of L-arg (10-2 M) alone did not affect baseline Q(LDF). Also, L-arg did not influence the ACh-induced vasodilating response, whereas it slightly increased the SNP-induced vasodilating response. These data suggest that the increase in endothelium-dependent blood flow stimulated by agonists may occur independently of low baseline EDRF activity in the diaphragmatic microvascular bed of anesthetized rats.

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