Differential proteomic analysis of human placenta-derived mesenchymal stem cells cultured on normal tissue culture surface and hyaluronan-coated surface

Tzyy Yue Wong, Ying Hui Chen, Szu Heng Liu, Mairim Alexandra Solis, Chen-Hsiang Yu, Chiung-Hsin Chang, Lynn Ling-Huei Huang

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Our previous results showed that hyaluronan (HA) preserved human placenta-derived mesenchymal stem cells (PDMSC) in a slow cell cycling mode similar to quiescence, the pristine state of stem cells in vivo, and HA was found to prevent murine adipose-derived mesenchymal stem cells from senescence. Here, stable isotope labeling by amino acid in cell culture (SILAC) proteomic profiling was used to evaluate the effects of HA on aging phenomenon in stem cells, comparing (1) old and young passage PDMSC cultured on normal tissue culture surface (TCS); (2) old passage on HA-coated surface (CHA) compared to TCS; (3) old and young passage on CHA. The results indicated that senescence-associated protein transgelin (TAGLN) was upregulated in old TCS. Protein CYR61, reportedly senescence-related, was downregulated in old CHA compared to old TCS. The SIRT1-interacting Nicotinamide phosphoribosyltransferase (NAMPT) increased by 2.23-fold in old CHA compared to old TCS, and is 0.48-fold lower in old TCS compared to young TCS. Results also indicated that components of endoplasmic reticulum associated degradation (ERAD) pathway were upregulated in old CHA compared to old TCS cells, potentially for overcoming stress to maintain cell function and suppress senescence. Our data points to pathways that may be targeted by HA to maintain stem cells youth.

Original languageEnglish
Article number2809192
JournalStem Cells International
Volume2016
DOIs
Publication statusPublished - 2016 Jan 1

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Hyaluronic Acid
Mesenchymal Stromal Cells
Proteomics
Placenta
Stem Cells
Cysteine-Rich Protein 61
Cell Culture Techniques
Endoplasmic Reticulum-Associated Degradation
Nicotinamide Phosphoribosyltransferase
Isotope Labeling
Cell Aging
Down-Regulation
Amino Acids

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

Cite this

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abstract = "Our previous results showed that hyaluronan (HA) preserved human placenta-derived mesenchymal stem cells (PDMSC) in a slow cell cycling mode similar to quiescence, the pristine state of stem cells in vivo, and HA was found to prevent murine adipose-derived mesenchymal stem cells from senescence. Here, stable isotope labeling by amino acid in cell culture (SILAC) proteomic profiling was used to evaluate the effects of HA on aging phenomenon in stem cells, comparing (1) old and young passage PDMSC cultured on normal tissue culture surface (TCS); (2) old passage on HA-coated surface (CHA) compared to TCS; (3) old and young passage on CHA. The results indicated that senescence-associated protein transgelin (TAGLN) was upregulated in old TCS. Protein CYR61, reportedly senescence-related, was downregulated in old CHA compared to old TCS. The SIRT1-interacting Nicotinamide phosphoribosyltransferase (NAMPT) increased by 2.23-fold in old CHA compared to old TCS, and is 0.48-fold lower in old TCS compared to young TCS. Results also indicated that components of endoplasmic reticulum associated degradation (ERAD) pathway were upregulated in old CHA compared to old TCS cells, potentially for overcoming stress to maintain cell function and suppress senescence. Our data points to pathways that may be targeted by HA to maintain stem cells youth.",
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Differential proteomic analysis of human placenta-derived mesenchymal stem cells cultured on normal tissue culture surface and hyaluronan-coated surface. / Wong, Tzyy Yue; Chen, Ying Hui; Liu, Szu Heng; Solis, Mairim Alexandra; Yu, Chen-Hsiang; Chang, Chiung-Hsin; Huang, Lynn Ling-Huei.

In: Stem Cells International, Vol. 2016, 2809192, 01.01.2016.

Research output: Contribution to journalArticle

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AU - Wong, Tzyy Yue

AU - Chen, Ying Hui

AU - Liu, Szu Heng

AU - Solis, Mairim Alexandra

AU - Yu, Chen-Hsiang

AU - Chang, Chiung-Hsin

AU - Huang, Lynn Ling-Huei

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