Differential proteomic characterization between normal peritoneal fluid and diabetic peritoneal dialysate

Hsien Yi Wang, Yu Feng Tian, Chih Chiang Chien, Wei Chih Kan, Pao Chi Liao, Hsin Yi Wu, Shih Bin Su, Ching Yih Lin

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Background. Since the mechanism of comorbidity and mortality in peritoneal dialysis is unclear, a comparison of peritoneal dialysate and normal peritoneal fluid may provide clues to the biological and pathological processes involved in peritoneal damage.Methods. Peritoneal dialysate and control samples were collected from five diabetes mellitus (DM) patients and two patients receiving laparoscopic cholecystectomy. Proteins were separated by two-dimensional gel electrophoresis (2D-GE). After image analysis, altered gel spots between these two sample groups were subjected to tryptic digestion and mass spectrometry analysis. The results were searched against the NCBI database.Results. A total of 26 protein spots were considered altered in 2D-GE between the two sample groups. After western blotting confirmation, vitamin D-binding protein, haptoglobin and α-2-microglobulin were at higher levels in the DM samples, while complement C4-A and IGK@ protein were at lower levels compared to the control samples.Conclusion. The loss of vitamin D-binding protein, haptoglobin and α-2-microglobulin may be due to a change in the permeability of the peritoneal membrane to middle-sized proteins or leakage from peritoneal inflammation. Lower levels of complement C4-A in dialysate may shed light on the beginning of peritoneal membrane scleroses.

Original languageEnglish
Pages (from-to)1955-1963
Number of pages9
JournalNephrology Dialysis Transplantation
Volume25
Issue number6
DOIs
Publication statusPublished - 2010 Jun 1

All Science Journal Classification (ASJC) codes

  • Nephrology
  • Transplantation

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