Diffuse optical spectroscopy of melanoma-simulating silicone phantoms

Alexander M. Grant, Kelly Sry, Kelly Saager, Frederick Ayers, T. Joshua Pfefer, Kristen M. Kelly, Sheng-Hao Tseng, Anthony J. Durkin

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Citations (Scopus)

Abstract

Currently the only method for positively identifying malignant melanoma involves invasive and often undesirable biopsy procedures. Available ex-vivo data indicates increased vascularization in the lower regions of excised melanoma, as compared to dysplastic nevi. The ability to interrogate this region of tissue in-vivo could lead to useful diagnostic information. Using a newly developed fiber based superficial probe in conjunction with a steady-state frequency-domain photon migration (SSFDPM) system, we can probe the upper 1-2 mm of tissue, extracting functional information in the near infrared (650-1000 nm) range. To test the resolution and detection range of the superficial probe in this context,deformable silicone phantoms have been fabricated that simulate normal skin with melanocytic lesions. These phantoms consist of a two-layered matrix with the optical properties of normal light skin, containing several cylindrical inclusions that simulate highly absorbing pigmented lesions such as melanoma. The e inclusions are varied in depth, diameter, and optical properties in order to fully test the probe's detection capabilities. It was found that, depending on absorption, we can typically probe to a depth of 1.0-1.5 mm in an inclusion, likely reaching the site of angiogenesis in an early-stage melanoma. Additionally, we can successfully interrogate normal tissue below lesions 1.5mm deep when absorption is about 0.4/mm or less. This data indicates that the superficial probe shows great promise for non-invasive diagnosis of pigmented lesions.

Original languageEnglish
Title of host publicationBiomedical Applications of Light Scattering III
DOIs
Publication statusPublished - 2009 Jun 12
EventBiomedical Applications of Light Scattering III - San Jose, CA, United States
Duration: 2009 Jan 242009 Jan 26

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume7187
ISSN (Print)1605-7422

Other

OtherBiomedical Applications of Light Scattering III
CountryUnited States
CitySan Jose, CA
Period09-01-2409-01-26

Fingerprint

silicones
Silicones
Melanoma
Spectrum Analysis
lesions
probes
spectroscopy
Dysplastic Nevus Syndrome
inclusions
Tissue
Skin
Optical properties
Photons
optical properties
angiogenesis
Biopsy
Light
Optical spectroscopy
Infrared radiation
fibers

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

Cite this

Grant, A. M., Sry, K., Saager, K., Ayers, F., Pfefer, T. J., Kelly, K. M., ... Durkin, A. J. (2009). Diffuse optical spectroscopy of melanoma-simulating silicone phantoms. In Biomedical Applications of Light Scattering III [718702] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 7187). https://doi.org/10.1117/12.809488
Grant, Alexander M. ; Sry, Kelly ; Saager, Kelly ; Ayers, Frederick ; Pfefer, T. Joshua ; Kelly, Kristen M. ; Tseng, Sheng-Hao ; Durkin, Anthony J. / Diffuse optical spectroscopy of melanoma-simulating silicone phantoms. Biomedical Applications of Light Scattering III. 2009. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE).
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title = "Diffuse optical spectroscopy of melanoma-simulating silicone phantoms",
abstract = "Currently the only method for positively identifying malignant melanoma involves invasive and often undesirable biopsy procedures. Available ex-vivo data indicates increased vascularization in the lower regions of excised melanoma, as compared to dysplastic nevi. The ability to interrogate this region of tissue in-vivo could lead to useful diagnostic information. Using a newly developed fiber based superficial probe in conjunction with a steady-state frequency-domain photon migration (SSFDPM) system, we can probe the upper 1-2 mm of tissue, extracting functional information in the near infrared (650-1000 nm) range. To test the resolution and detection range of the superficial probe in this context,deformable silicone phantoms have been fabricated that simulate normal skin with melanocytic lesions. These phantoms consist of a two-layered matrix with the optical properties of normal light skin, containing several cylindrical inclusions that simulate highly absorbing pigmented lesions such as melanoma. The e inclusions are varied in depth, diameter, and optical properties in order to fully test the probe's detection capabilities. It was found that, depending on absorption, we can typically probe to a depth of 1.0-1.5 mm in an inclusion, likely reaching the site of angiogenesis in an early-stage melanoma. Additionally, we can successfully interrogate normal tissue below lesions 1.5mm deep when absorption is about 0.4/mm or less. This data indicates that the superficial probe shows great promise for non-invasive diagnosis of pigmented lesions.",
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Grant, AM, Sry, K, Saager, K, Ayers, F, Pfefer, TJ, Kelly, KM, Tseng, S-H & Durkin, AJ 2009, Diffuse optical spectroscopy of melanoma-simulating silicone phantoms. in Biomedical Applications of Light Scattering III., 718702, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 7187, Biomedical Applications of Light Scattering III, San Jose, CA, United States, 09-01-24. https://doi.org/10.1117/12.809488

Diffuse optical spectroscopy of melanoma-simulating silicone phantoms. / Grant, Alexander M.; Sry, Kelly; Saager, Kelly; Ayers, Frederick; Pfefer, T. Joshua; Kelly, Kristen M.; Tseng, Sheng-Hao; Durkin, Anthony J.

Biomedical Applications of Light Scattering III. 2009. 718702 (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 7187).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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Grant AM, Sry K, Saager K, Ayers F, Pfefer TJ, Kelly KM et al. Diffuse optical spectroscopy of melanoma-simulating silicone phantoms. In Biomedical Applications of Light Scattering III. 2009. 718702. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE). https://doi.org/10.1117/12.809488