Direct binding of Concanvalin A onto iron oxide nanoparticles for fast magnetic selective separation of lactoferrin

Bo Hung Lai, Chih Hao Chang, Chun Chieh Yeh, Dong-Hwang Chen

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Concanavalin A (Con A) was successfully bound onto Fe3O 4 magnetic nanoparticles (MNPs) via carbodiimide activation as a magnetic nano-adsorbent for glycoprotein separation. The resulting Con A-bound Fe3O4 MNPs were nearly superparamagnetic with a mean diameter of 11.74 ± 3.86 nm. The size and structure of Fe 3O4 MNPs had no significant changes after Con A binding. The binding of Con A on the surface of Fe3O4 MNPs was confirmed by the analysis of Fourier transform infrared spectroscopy. From the thermogravimetric analysis and the concentration change of Con A in the binding solution, the weight percentage of Con A in the typical Con A-bound Fe 3O4 MNPs was determined to be about 10 wt%. Furthermore, the Con A-bound Fe3O4 MNPs were found to be quite efficient for the fast adsorption of lactoferrin. The equilibrium was achieved within 5 min and the adsorption data for lactoferrin obeyed the Langmuir isotherm equation. At pH 7 and 25 °C, the maximum adsorption capacity and equilibrium constant were 59.2 mg/g and 0.0103 L/mg, respectively. In addition, by SDS-polyacrylamide gel electrophoresis, the capability of Con A-bound Fe 3O4 MNPs for the selective adsorption of lactoferrin was further confirmed.

Original languageEnglish
Pages (from-to)83-88
Number of pages6
JournalSeparation and Purification Technology
Volume108
DOIs
Publication statusPublished - 2013 Mar 15

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Lactoferrin
Concanavalin A
Iron oxides
Nanoparticles
Adsorption
Glycoproteins
Equilibrium constants
ferric oxide
Carbodiimides
Polyacrylates
Electrophoresis
Adsorbents
Fourier transform infrared spectroscopy
Isotherms
Thermogravimetric analysis
Gels
Chemical activation

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Filtration and Separation

Cite this

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title = "Direct binding of Concanvalin A onto iron oxide nanoparticles for fast magnetic selective separation of lactoferrin",
abstract = "Concanavalin A (Con A) was successfully bound onto Fe3O 4 magnetic nanoparticles (MNPs) via carbodiimide activation as a magnetic nano-adsorbent for glycoprotein separation. The resulting Con A-bound Fe3O4 MNPs were nearly superparamagnetic with a mean diameter of 11.74 ± 3.86 nm. The size and structure of Fe 3O4 MNPs had no significant changes after Con A binding. The binding of Con A on the surface of Fe3O4 MNPs was confirmed by the analysis of Fourier transform infrared spectroscopy. From the thermogravimetric analysis and the concentration change of Con A in the binding solution, the weight percentage of Con A in the typical Con A-bound Fe 3O4 MNPs was determined to be about 10 wt{\%}. Furthermore, the Con A-bound Fe3O4 MNPs were found to be quite efficient for the fast adsorption of lactoferrin. The equilibrium was achieved within 5 min and the adsorption data for lactoferrin obeyed the Langmuir isotherm equation. At pH 7 and 25 °C, the maximum adsorption capacity and equilibrium constant were 59.2 mg/g and 0.0103 L/mg, respectively. In addition, by SDS-polyacrylamide gel electrophoresis, the capability of Con A-bound Fe 3O4 MNPs for the selective adsorption of lactoferrin was further confirmed.",
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Direct binding of Concanvalin A onto iron oxide nanoparticles for fast magnetic selective separation of lactoferrin. / Lai, Bo Hung; Chang, Chih Hao; Yeh, Chun Chieh; Chen, Dong-Hwang.

In: Separation and Purification Technology, Vol. 108, 15.03.2013, p. 83-88.

Research output: Contribution to journalArticle

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PY - 2013/3/15

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AB - Concanavalin A (Con A) was successfully bound onto Fe3O 4 magnetic nanoparticles (MNPs) via carbodiimide activation as a magnetic nano-adsorbent for glycoprotein separation. The resulting Con A-bound Fe3O4 MNPs were nearly superparamagnetic with a mean diameter of 11.74 ± 3.86 nm. The size and structure of Fe 3O4 MNPs had no significant changes after Con A binding. The binding of Con A on the surface of Fe3O4 MNPs was confirmed by the analysis of Fourier transform infrared spectroscopy. From the thermogravimetric analysis and the concentration change of Con A in the binding solution, the weight percentage of Con A in the typical Con A-bound Fe 3O4 MNPs was determined to be about 10 wt%. Furthermore, the Con A-bound Fe3O4 MNPs were found to be quite efficient for the fast adsorption of lactoferrin. The equilibrium was achieved within 5 min and the adsorption data for lactoferrin obeyed the Langmuir isotherm equation. At pH 7 and 25 °C, the maximum adsorption capacity and equilibrium constant were 59.2 mg/g and 0.0103 L/mg, respectively. In addition, by SDS-polyacrylamide gel electrophoresis, the capability of Con A-bound Fe 3O4 MNPs for the selective adsorption of lactoferrin was further confirmed.

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