Direct detection of virus-like particles using color images of plasmonic nanostructures

Yi Ru Li, Kuang Li Lee, Kuan Ming Chen, Yun Cheng Lu, Pin Chieh Wu, Sy Hann Chen, Jiun Haw Lee, Pei Kuen Wei

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)


We propose a measurement method for sensitive and label-free detections of virus-like particles (VLPs) using color images of nanoplasmonic sensing chips. The nanoplasmonic chip consists of 5×5 gold nanoslit arrays and the gold surface is modified with specific antibodies for spike protein. The resonant wavelength of the 430-nm-period gold nanoslit arrays underwater environment is about 570 nm which falls between the green and red bands of the color CCD. The captured VLPs by the specific antibodies shift the plasmonic resonance of the gold nanoslits. It results in an increased brightness of green pixels and decreased brightness of red pixels. The image contrast signals of (green - red) / (red+green) show good linearity with the surface particle density. The experimental tests show the image contrast method can detect 100-nm polystyrene particles with a surface density smaller than 2 particles/μm2. We demonstrate the application for direct detection of SARS-CoV-2 VLPs using a simple scanner platform. A detection limit smaller than 1 pg/mL with a detection time less than 30 minutes can be achieved.

Original languageEnglish
Pages (from-to)22233-22246
Number of pages14
JournalOptics Express
Issue number12
Publication statusPublished - 2022 Jun 6

All Science Journal Classification (ASJC) codes

  • Atomic and Molecular Physics, and Optics


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