TY - JOUR
T1 - Direct electron transfer of glucose oxidase and dual hydrogen peroxide and glucose detection based on water-dispersible carbon nanotubes derivative
AU - Chen, Hsiao Chien
AU - Tu, Yi Ming
AU - Hou, Chung Che
AU - Lin, Yu Chen
AU - Chen, Ching Hsiang
AU - Yang, Kuang Hsuan
N1 - Funding Information:
We thank the Vanung University and Ministry of Science and Technology (MOST) of ROC for financial aid: NSC 102-2622-E-238-010-CC3 and MOST 103-2221-E-238-006 .
Publisher Copyright:
© 2015.
PY - 2015/3/31
Y1 - 2015/3/31
N2 - A water-dispersible multi-walled carbon nanotubes (MWCNTs) derivative, MWCNTs-1-one-dihydroxypyridine (MWCNTs-Py) was synthesis via Friedel-Crafts chemical acylation. Raman spectra demonstrated the conjugated level of MWCNTs-Py was retained after this chemical modification. MWCNTs-Py showed dual hydrogen peroxide (H2O2) and glucose detections without mutual interference by adjusting pH value. It was sensitive to H2O2 in acidic solution and displayed the high performances of sensitivity, linear range, response time and stability; meanwhile it did not respond to H2O2 in neutral solution. In addition, this positively charged MWCNTs-Py could adsorb glucose oxidase (GOD) by electrostatic attraction. MWCNTs-Py-GOD/GC electrode showed the direct electron transfer (DET) of GOD with a pair of well-defined redox peaks, attesting the bioactivity of GOD was retained due to the non-destroyed immobilization. The high surface coverage of active GOD (3.5×10-9molcm-2) resulted in exhibiting a good electrocatalytic activity toward glucose. This glucose sensor showed high sensitivity (68.1μAmM-1cm-2) in a linear range from 3μM to 7mM in neutral buffer solution. The proposed sensor could distinguish H2O2 and glucose, thus owning high selectivity and reliability.
AB - A water-dispersible multi-walled carbon nanotubes (MWCNTs) derivative, MWCNTs-1-one-dihydroxypyridine (MWCNTs-Py) was synthesis via Friedel-Crafts chemical acylation. Raman spectra demonstrated the conjugated level of MWCNTs-Py was retained after this chemical modification. MWCNTs-Py showed dual hydrogen peroxide (H2O2) and glucose detections without mutual interference by adjusting pH value. It was sensitive to H2O2 in acidic solution and displayed the high performances of sensitivity, linear range, response time and stability; meanwhile it did not respond to H2O2 in neutral solution. In addition, this positively charged MWCNTs-Py could adsorb glucose oxidase (GOD) by electrostatic attraction. MWCNTs-Py-GOD/GC electrode showed the direct electron transfer (DET) of GOD with a pair of well-defined redox peaks, attesting the bioactivity of GOD was retained due to the non-destroyed immobilization. The high surface coverage of active GOD (3.5×10-9molcm-2) resulted in exhibiting a good electrocatalytic activity toward glucose. This glucose sensor showed high sensitivity (68.1μAmM-1cm-2) in a linear range from 3μM to 7mM in neutral buffer solution. The proposed sensor could distinguish H2O2 and glucose, thus owning high selectivity and reliability.
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U2 - 10.1016/j.aca.2015.01.027
DO - 10.1016/j.aca.2015.01.027
M3 - Article
C2 - 25813031
AN - SCOPUS:84925246206
SN - 0003-2670
VL - 867
SP - 83
EP - 91
JO - Analytica Chimica Acta
JF - Analytica Chimica Acta
ER -