Direct examination of separation processes in liquid chromatography

Effect of temperature and pressure on solute retention

Victoria L. McGuffin, Christine E. Evans, Shu-Hui Chen

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

In this study, retention processes in reversed‐phase liquid chromatography were examined in situ along an optically transparent column by means of laser‐induced fluorescence. Using a homologous series of derivatized fatty acids as model solutes, the capacity factors were systematically measured as a function of temperature in the range from 10 to 60°C and pressure in the range from 980 to 4,430 psi. A phase transition was observed for the octadecylsilica stationary phase as temperature was decreased and as pressure was increased. From evaluation of its thermodynamic properties during this phase transition, the stationary phase appears to be more similar to a randomly oriented alkane solvent than a highly ordered system such as a phospholipid membrane. Because this phase transition occurs within the range of routine operating conditions in liquid chromatography, it has important implications for both fundamental and practical applications.

Original languageEnglish
Pages (from-to)3-10
Number of pages8
JournalJournal of Microcolumn Separations
Volume5
Issue number1
DOIs
Publication statusPublished - 1993 Jan 1

Fingerprint

Liquid chromatography
Phase transitions
Alkanes
Phospholipids
Fatty acids
Paraffins
Temperature
Fatty Acids
Thermodynamic properties
Fluorescence
Membranes

All Science Journal Classification (ASJC) codes

  • Mechanical Engineering
  • Filtration and Separation

Cite this

@article{017feb924b904bb7b6cb9289b83d0783,
title = "Direct examination of separation processes in liquid chromatography: Effect of temperature and pressure on solute retention",
abstract = "In this study, retention processes in reversed‐phase liquid chromatography were examined in situ along an optically transparent column by means of laser‐induced fluorescence. Using a homologous series of derivatized fatty acids as model solutes, the capacity factors were systematically measured as a function of temperature in the range from 10 to 60°C and pressure in the range from 980 to 4,430 psi. A phase transition was observed for the octadecylsilica stationary phase as temperature was decreased and as pressure was increased. From evaluation of its thermodynamic properties during this phase transition, the stationary phase appears to be more similar to a randomly oriented alkane solvent than a highly ordered system such as a phospholipid membrane. Because this phase transition occurs within the range of routine operating conditions in liquid chromatography, it has important implications for both fundamental and practical applications.",
author = "McGuffin, {Victoria L.} and Evans, {Christine E.} and Shu-Hui Chen",
year = "1993",
month = "1",
day = "1",
doi = "10.1002/mcs.1220050102",
language = "English",
volume = "5",
pages = "3--10",
journal = "Journal of Microcolumn Separations",
issn = "1040-7685",
publisher = "John Wiley and Sons Ltd",
number = "1",

}

Direct examination of separation processes in liquid chromatography : Effect of temperature and pressure on solute retention. / McGuffin, Victoria L.; Evans, Christine E.; Chen, Shu-Hui.

In: Journal of Microcolumn Separations, Vol. 5, No. 1, 01.01.1993, p. 3-10.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Direct examination of separation processes in liquid chromatography

T2 - Effect of temperature and pressure on solute retention

AU - McGuffin, Victoria L.

AU - Evans, Christine E.

AU - Chen, Shu-Hui

PY - 1993/1/1

Y1 - 1993/1/1

N2 - In this study, retention processes in reversed‐phase liquid chromatography were examined in situ along an optically transparent column by means of laser‐induced fluorescence. Using a homologous series of derivatized fatty acids as model solutes, the capacity factors were systematically measured as a function of temperature in the range from 10 to 60°C and pressure in the range from 980 to 4,430 psi. A phase transition was observed for the octadecylsilica stationary phase as temperature was decreased and as pressure was increased. From evaluation of its thermodynamic properties during this phase transition, the stationary phase appears to be more similar to a randomly oriented alkane solvent than a highly ordered system such as a phospholipid membrane. Because this phase transition occurs within the range of routine operating conditions in liquid chromatography, it has important implications for both fundamental and practical applications.

AB - In this study, retention processes in reversed‐phase liquid chromatography were examined in situ along an optically transparent column by means of laser‐induced fluorescence. Using a homologous series of derivatized fatty acids as model solutes, the capacity factors were systematically measured as a function of temperature in the range from 10 to 60°C and pressure in the range from 980 to 4,430 psi. A phase transition was observed for the octadecylsilica stationary phase as temperature was decreased and as pressure was increased. From evaluation of its thermodynamic properties during this phase transition, the stationary phase appears to be more similar to a randomly oriented alkane solvent than a highly ordered system such as a phospholipid membrane. Because this phase transition occurs within the range of routine operating conditions in liquid chromatography, it has important implications for both fundamental and practical applications.

UR - http://www.scopus.com/inward/record.url?scp=84986772909&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84986772909&partnerID=8YFLogxK

U2 - 10.1002/mcs.1220050102

DO - 10.1002/mcs.1220050102

M3 - Article

VL - 5

SP - 3

EP - 10

JO - Journal of Microcolumn Separations

JF - Journal of Microcolumn Separations

SN - 1040-7685

IS - 1

ER -