Direct interaction of C/EBPδ and Sp1 at the GC-enriched promoter region synergizes the IL-10 gene transcription in mouse macrophage

Ben Tzu Chiang, Yi Wen Liu, Ben-Kuen Chen, Ju-Ming Wang, Wen Chang Chang

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

We previously reported that LPS activates the transcription of the IL-10 gene through the Sp1 and C/EBP binding sites and indicated that Sp1, C/EBPβ and C/EBPδ can coactivate the IL-10 gene expression in mouse macrophage cells [Liu Y.-W., Tseng H.-P., Chen L.-C., Chen B.-K., Chang W.-C. J. Immunol. 171: 821-828, 2003]. In the present report, we demonstrated the direct physical interaction between C/EBPδ and Sp1, and also mapped the interaction domains of these two proteins. C/EBPδ binds to Sp1 via its basic region leucine zipper domain. The C-terminus of Sp1 was also the major region interacting with C/EBPδ. However, both glutamine- and serine/threonine- rich homologus regions of Sp1 also interacted with C/EBPδ. The binding of Sp1 and C/EBPδ as a complex to the Sp1 binding site on the promoter of IL-10 was further confirmed by using the DNA affinity precipitation assay. By using Sp1-deficient SL2 cells, we also found that the overexpressions of C/EBPδ and Sp1 synergically activate the transcriptional activity of IL-10 gene. Taken together, our present results revealed a novel mechanism of a superactivation of Sp1 by C/EBPδ via a direct interaction between these two transcription factors leading to the activation of the IL-10 gene in mouse macrophage cells.

Original languageEnglish
Pages (from-to)621-635
Number of pages15
JournalJournal of biomedical science
Volume13
Issue number5
DOIs
Publication statusPublished - 2006 Sep 1

Fingerprint

Macrophages
Transcription
Genetic Promoter Regions
Interleukin-10
Genes
Binding Sites
Protein Interaction Domains and Motifs
Leucine Zippers
Threonine
Glutamine
Gene expression
Serine
Assays
Transcription Factors
Chemical activation
Gene Expression
DNA
Proteins

All Science Journal Classification (ASJC) codes

  • Endocrinology, Diabetes and Metabolism
  • Molecular Biology
  • Clinical Biochemistry
  • Cell Biology
  • Biochemistry, medical
  • Pharmacology (medical)

Cite this

@article{81ebe5621da04bf490aa3b11f18e92ef,
title = "Direct interaction of C/EBPδ and Sp1 at the GC-enriched promoter region synergizes the IL-10 gene transcription in mouse macrophage",
abstract = "We previously reported that LPS activates the transcription of the IL-10 gene through the Sp1 and C/EBP binding sites and indicated that Sp1, C/EBPβ and C/EBPδ can coactivate the IL-10 gene expression in mouse macrophage cells [Liu Y.-W., Tseng H.-P., Chen L.-C., Chen B.-K., Chang W.-C. J. Immunol. 171: 821-828, 2003]. In the present report, we demonstrated the direct physical interaction between C/EBPδ and Sp1, and also mapped the interaction domains of these two proteins. C/EBPδ binds to Sp1 via its basic region leucine zipper domain. The C-terminus of Sp1 was also the major region interacting with C/EBPδ. However, both glutamine- and serine/threonine- rich homologus regions of Sp1 also interacted with C/EBPδ. The binding of Sp1 and C/EBPδ as a complex to the Sp1 binding site on the promoter of IL-10 was further confirmed by using the DNA affinity precipitation assay. By using Sp1-deficient SL2 cells, we also found that the overexpressions of C/EBPδ and Sp1 synergically activate the transcriptional activity of IL-10 gene. Taken together, our present results revealed a novel mechanism of a superactivation of Sp1 by C/EBPδ via a direct interaction between these two transcription factors leading to the activation of the IL-10 gene in mouse macrophage cells.",
author = "Chiang, {Ben Tzu} and Liu, {Yi Wen} and Ben-Kuen Chen and Ju-Ming Wang and Chang, {Wen Chang}",
year = "2006",
month = "9",
day = "1",
doi = "10.1007/s11373-006-9101-y",
language = "English",
volume = "13",
pages = "621--635",
journal = "Journal of Biomedical Science",
issn = "1021-7770",
publisher = "BioMed Central",
number = "5",

}

Direct interaction of C/EBPδ and Sp1 at the GC-enriched promoter region synergizes the IL-10 gene transcription in mouse macrophage. / Chiang, Ben Tzu; Liu, Yi Wen; Chen, Ben-Kuen; Wang, Ju-Ming; Chang, Wen Chang.

In: Journal of biomedical science, Vol. 13, No. 5, 01.09.2006, p. 621-635.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Direct interaction of C/EBPδ and Sp1 at the GC-enriched promoter region synergizes the IL-10 gene transcription in mouse macrophage

AU - Chiang, Ben Tzu

AU - Liu, Yi Wen

AU - Chen, Ben-Kuen

AU - Wang, Ju-Ming

AU - Chang, Wen Chang

PY - 2006/9/1

Y1 - 2006/9/1

N2 - We previously reported that LPS activates the transcription of the IL-10 gene through the Sp1 and C/EBP binding sites and indicated that Sp1, C/EBPβ and C/EBPδ can coactivate the IL-10 gene expression in mouse macrophage cells [Liu Y.-W., Tseng H.-P., Chen L.-C., Chen B.-K., Chang W.-C. J. Immunol. 171: 821-828, 2003]. In the present report, we demonstrated the direct physical interaction between C/EBPδ and Sp1, and also mapped the interaction domains of these two proteins. C/EBPδ binds to Sp1 via its basic region leucine zipper domain. The C-terminus of Sp1 was also the major region interacting with C/EBPδ. However, both glutamine- and serine/threonine- rich homologus regions of Sp1 also interacted with C/EBPδ. The binding of Sp1 and C/EBPδ as a complex to the Sp1 binding site on the promoter of IL-10 was further confirmed by using the DNA affinity precipitation assay. By using Sp1-deficient SL2 cells, we also found that the overexpressions of C/EBPδ and Sp1 synergically activate the transcriptional activity of IL-10 gene. Taken together, our present results revealed a novel mechanism of a superactivation of Sp1 by C/EBPδ via a direct interaction between these two transcription factors leading to the activation of the IL-10 gene in mouse macrophage cells.

AB - We previously reported that LPS activates the transcription of the IL-10 gene through the Sp1 and C/EBP binding sites and indicated that Sp1, C/EBPβ and C/EBPδ can coactivate the IL-10 gene expression in mouse macrophage cells [Liu Y.-W., Tseng H.-P., Chen L.-C., Chen B.-K., Chang W.-C. J. Immunol. 171: 821-828, 2003]. In the present report, we demonstrated the direct physical interaction between C/EBPδ and Sp1, and also mapped the interaction domains of these two proteins. C/EBPδ binds to Sp1 via its basic region leucine zipper domain. The C-terminus of Sp1 was also the major region interacting with C/EBPδ. However, both glutamine- and serine/threonine- rich homologus regions of Sp1 also interacted with C/EBPδ. The binding of Sp1 and C/EBPδ as a complex to the Sp1 binding site on the promoter of IL-10 was further confirmed by using the DNA affinity precipitation assay. By using Sp1-deficient SL2 cells, we also found that the overexpressions of C/EBPδ and Sp1 synergically activate the transcriptional activity of IL-10 gene. Taken together, our present results revealed a novel mechanism of a superactivation of Sp1 by C/EBPδ via a direct interaction between these two transcription factors leading to the activation of the IL-10 gene in mouse macrophage cells.

UR - http://www.scopus.com/inward/record.url?scp=33749343200&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33749343200&partnerID=8YFLogxK

U2 - 10.1007/s11373-006-9101-y

DO - 10.1007/s11373-006-9101-y

M3 - Article

VL - 13

SP - 621

EP - 635

JO - Journal of Biomedical Science

JF - Journal of Biomedical Science

SN - 1021-7770

IS - 5

ER -