Direct measurement of the rupture force of single pair of decorin interactions

Xuhui Liu, Ming Long Yeh, Jack L. Lewis, Zong Ping Luo

Research output: Contribution to journalArticlepeer-review

24 Citations (Scopus)

Abstract

Decorin is one important member of the family of small leucine-rich proteoglycans, which are widely distributed in connective tissues in the body such as tendon and ligament. Decorin may be responsible for collagen fibril connection in those tissues. A recent hypothesis suggests that decorin may bind to collagen with its core protein while binding to another decorin through the interaction with their glycosaminoglycan (GAG) chains. However, there is no direct evidence supporting this hypothesis to date. In this study, the interaction of decorin GAG chains was directly determined for the first time. The rupture force of single bonds between decorins (GAG chains interaction) was determined directly as 16.5 ± 5.1 pN using a laser tweezers/ interferometer single molecular nanomechanical testing system. This information can improve our understanding of the mechanical properties of connective tissues at the molecular level.

Original languageEnglish
Pages (from-to)1342-1345
Number of pages4
JournalBiochemical and Biophysical Research Communications
Volume338
Issue number3
DOIs
Publication statusPublished - 2005 Dec 23

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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