Discovery of retinoblastoma-associated binding protein 46 as a novel prognostic marker for distant metastasis in nonsmall cell lung cancer by combined analysis of cancer cell secretome and pleural effusion proteome

Chih Liang Wang, Chun I. Wang, Pao Chi Liao, Chi De Chen, Ying Liang, Wen Yu Chuang, Ying Huang Tsai, Hua Chien Chen, Yu Sun Chang, Jau Song Yu, Chih Ching Wu, Chia Jung Yu

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51 Citations (Scopus)

Abstract

Nonsmall cell lung cancer (NSCLC) is the most common type of lung cancer, which is one of the most prominent causes of cancer-related mortality worldwide. Discovery of serum tumor markers could facilitate early NSCLC detection and metastatic prognosis. Here, we simultaneously analyzed the NSCLC cell secretome and proteomic profiles of pleural effusion from lung adenocarcinoma patients for NSCLC biomarker discovery. Retinoblastoma-associated binding protein 46 (RbAp46), one of the proteins detected both in NSCLC cell secretome and pleural effusion proteome, was chosen for further evaluation. Both of RbAp46 mRNA and protein levels were upregulated significantly in NSCLC cancer tissues. Serum levels of RbAp46 were markedly higher in NSCLC patients than in healthy controls, and a combination of RbAp46 and CEA could outperform CEA alone in discriminating NSCLC patients from healthy persons. Importantly, elevated serum RbAp46 level was highly correlated with NSCLC distant metastasis. Moreover, knockdown of RbAp46 inhibited the migration ability of lung cancer cells. Our data collectively suggest that RbAp46 serves as a novel biomarker and prognosticator for NSCLC, and is involved in lung cancer cell migration.

Original languageEnglish
Pages (from-to)4428-4440
Number of pages13
JournalJournal of Proteome Research
Volume8
Issue number10
DOIs
Publication statusPublished - 2009 Oct 19

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All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Chemistry(all)

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