Distinct regulation of gene expression by prostaglandin F (PGF) is associated with PGF resistance or susceptibility in human granulosa-luteal cells

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

The effects of human chorionic gonadotrophin (HCG) and prostaglandin F (PGF) on regulation of human granulosa-luteal cell (GLC) function at different stages of differentiation (day 2 versus day 8 of culture) were studied. Expression of LH receptor mRNA and biosynthesis of progesterone were HCG dependent in human GLC at all stages (n=6,P < 0.05). Steady-state concentrations of mRNA encoding for FP (a specific high-affinity plasma membrane receptor for PGF) were not dependent on, but were stimulated by, addition of HCG (10 IU/ ml) or 8-bromo-cAMP (0.5 mmol/l) (n=6, P < 0.05). Treatment with PGF (100 nmol/l) decreased FP mRNA concentration, but had no effect on LH receptor and cyclo oxygenase-2 (COX-2) expression on day 2 of cultured GLC (n = 8). As a result, the progesterone biosynthesis by GLC was not affected. On day 8, PGF induced FP and PGHS-2 expression and at the same time decreased LH receptor expression, resulting in inhibition of progesterone output by GLC. Our data demonstrated that early stage GLC (day 2 of culture) are resistant to PGF-induced inhibition of progesterone synthesis but underwent further differentiation and acquired luteolytic capacity after 8 days culture in vitro. We conclude that, via distinct gene regulation at different stages of differentiation, human GLC may become resistant or susceptible to PGF-induced luteolysis.

Original languageEnglish
Pages (from-to)415-423
Number of pages9
JournalMolecular Human Reproduction
Volume7
Issue number5
DOIs
Publication statusPublished - 2001 May

All Science Journal Classification (ASJC) codes

  • Reproductive Medicine
  • Embryology
  • Molecular Biology
  • Genetics
  • Obstetrics and Gynaecology
  • Developmental Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'Distinct regulation of gene expression by prostaglandin F<sub>2α</sub> (PGF<sub>2α</sub>) is associated with PGF<sub>2α</sub> resistance or susceptibility in human granulosa-luteal cells'. Together they form a unique fingerprint.

Cite this