TY - JOUR
T1 - Distribution of vacuolar H+-pyrophosphatase and a membrane integral protein in a variety of green plants
AU - Maeshima, Masayoshi
AU - Mimura, Tetsuro
AU - Sato, Toshiyuki
N1 - Funding Information:
The authors wish to thank Dr. K. Altendorf of the University of Osnabrueck, Germany, for supplying bafilomycin A*. Young plants of Kalanchoe were kindly provided by Dr. T. Tezuka of the Nagoya University, and seeds of Arabidopsis by Drs. Y. Tanaka and H. Ichikawa of the National Institute of Agrobiological Resources, Tsukuba. This work was supported in part by Grants-in-Aid from the Ministry of Education, Science and culture of Japan to M.M.
PY - 1994
Y1 - 1994
N2 - Vacuolar membranes isolated from several species including fern and moss exhibited pyro-phosphate-dependent H+ transport activity. On immunoblot analysis, H+ -pyrophosphatase was detected in the vacuolar membranes. A membrane integral protein of 23,000 daltons was not found in the membranes of Chara, Conocephalum, or Kalanchoë. Thus, H+-pyrophosphatase may be a universal enzyme among green plants, but the 23-kDa protein is not a common protein of central vacuoles.
AB - Vacuolar membranes isolated from several species including fern and moss exhibited pyro-phosphate-dependent H+ transport activity. On immunoblot analysis, H+ -pyrophosphatase was detected in the vacuolar membranes. A membrane integral protein of 23,000 daltons was not found in the membranes of Chara, Conocephalum, or Kalanchoë. Thus, H+-pyrophosphatase may be a universal enzyme among green plants, but the 23-kDa protein is not a common protein of central vacuoles.
UR - https://www.scopus.com/pages/publications/0028137531
UR - https://www.scopus.com/pages/publications/0028137531#tab=citedBy
M3 - Article
AN - SCOPUS:0028137531
SN - 0032-0781
VL - 35
SP - 323
EP - 328
JO - Plant and Cell Physiology
JF - Plant and Cell Physiology
IS - 2
ER -