Divergent effects of cholesterol on the structure and fluidity of liposome and catanionic vesicle membranes

Chia Yu Cheng, Yu Fang Lai, Yu Ling Hsieh, Ching Hao Wu, Chi cheng Chiu, Yu Min Yang

Research output: Contribution to journalArticlepeer-review

Abstract

Lipid-like ion-pair amphiphile vesicles, or catanionic vesicles, have emerged as potential drug carriers. The effects of cholesterol on the properties of catanionic vesicles have yet been systematically studied. Here, we compared the effects of cholesterol on the structures and fluidities of dipalmitoylphosphatidylcholine liposomes and catanionic vesicles with similar main transition temperatures (Tm). For liposomes, fluorescence anisotropy (FA) thermograms reveal typical condensing and disordering effects of cholesterol above and below Tm respectively. In contrast, FA and molecular simulation data reveal that catanionic bilayers below Tm are more fluidic due to shorter alkyl chains. This leads to only condensing effects of cholesterol for catanionic vesicles at all temperatures. Our results provide important insights into the fabrication of catanionic vesicles as novel drug carriers.

Original languageEnglish
Pages (from-to)1827-1838
Number of pages12
JournalFEBS Letters
Volume596
Issue number14
DOIs
Publication statusPublished - 2022 Jul

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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