DNA-binding factors assemble in a sequence-specific manner on the maize mitochondrial atpA promoter

Ching Chun Chang, David B. Stern

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

The maize mitochondrial atpA promoter has been well-characterized using in vitro transcription. The functional elements of this promoter comprise a central domain extending from -7 to +5 relative to the transcription start site, and an upstream domain of 1-3 bp that is purine-rich and centered around positions -11 to -12. As a first step in characterizing the transcriptional machinery, exo-nuclease-III mapping (toeprinting) was used to map the borders of DNA-protein interactions using either a 107-bp wild-type template or transcriptionally-inactive templates containing linker-scanning mutations. These experiments revealed that, with a wild-type promoter, protein factors occupy as much as 36 bp, from positions -20 to +16 relative to the transcription initiation site. Protein-binding patterns were altered when the linker-scanning mutants were used, suggesting that either the number or conformation of DNA-binding proteins could account for their inability to promote transcription initiation.

Original languageEnglish
Pages (from-to)506-511
Number of pages6
JournalCurrent Genetics
Volume35
Issue number5
DOIs
Publication statusPublished - 1999

All Science Journal Classification (ASJC) codes

  • Genetics

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