Docosahexaenoic acid inhibits synaptic transmission and epileptiform activity in the rat hippocampus

Chainllie Young, Po-Wu Gean, Lih Chu Chiou, Yu Zen Shen

Research output: Contribution to journalArticle

72 Citations (Scopus)

Abstract

Docosahexaenoic acid (DHA) has been suggested to be required for neuronal development and synaptic plasticity. However, in view of the fact that DHA facilitates NMDA responses and blocks K+ channels, it might predispose the neurons to epileptiform bursting. By using extracellular recording of population spikes in the CA1 region of rat hippocampal slices, we tested this possibility by examining the effect of DHA on the epileptiform activity induced by bicuculline or in Mg2+-free medium. When stimuli were delivered to the Schaffer collateral/commissural pathway every 20 or 30 sec, DHA had no significant effect on the epileptiform activity. However, when the frequency of stimulation was increased to 0.2 Hz, DHA attenuated the amplitude of the bursting activity induced by bicuculline to 57.5 ± 10.8% and those induced by Mg2+-free ACSF to 65.8 ± 13.9% of control. DHA reduced the slope of field excitatory postsynaptic potential (fEPSP) to 77.1 ± 7.4% of baseline, without significant effect on the ratio of paired-pulse facilitation (PPF). By intracellular recording of neurons in the stratum pyramidale of rat hippocampal slices, we found that DHA markedly inhibited the repetitive firing of action potentials elicited by depolarizing current pulses but did not affect the initial action potential. Thus, DHA may attenuate epileptic activity mainly through the frequency-dependent blockade of Na+ channels. (C) 2000 Wiley-Liss, Inc.

Original languageEnglish
Pages (from-to)90-94
Number of pages5
JournalSynapse
Volume37
Issue number2
DOIs
Publication statusPublished - 2000 Jul 22

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Docosahexaenoic Acids
Synaptic Transmission
Hippocampus
Neuronal Plasticity
Bicuculline
Action Potentials
Hippocampal CA1 Region
Neurons
Excitatory Postsynaptic Potentials
N-Methylaspartate

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Physiology
  • Pharmacology

Cite this

Young, Chainllie ; Gean, Po-Wu ; Chiou, Lih Chu ; Shen, Yu Zen. / Docosahexaenoic acid inhibits synaptic transmission and epileptiform activity in the rat hippocampus. In: Synapse. 2000 ; Vol. 37, No. 2. pp. 90-94.
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Docosahexaenoic acid inhibits synaptic transmission and epileptiform activity in the rat hippocampus. / Young, Chainllie; Gean, Po-Wu; Chiou, Lih Chu; Shen, Yu Zen.

In: Synapse, Vol. 37, No. 2, 22.07.2000, p. 90-94.

Research output: Contribution to journalArticle

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