Dongshaea marina gen. Nov., sp. nov., a facultatively anaerobic marine bacterium that ferments glucose with gas production

Ssu Po Huang, Tzu Yin Chen, Jwo Sheng Chen, Li Ting Wang, Lina Huang, Shih Ting Lin, Chih Lin Wei, Saulwood Lin, Pei Ling Wang, Yi Min Chen, Wung Yang Shieh

Research output: Contribution to journalArticle

Abstract

Two isolates of heterotrophic, facultatively anaerobic, marine bacteria, designated DM1 and DM2T, were recovered from a lagoon sediment sample of Dongsha Island, Taiwan. Cells were Gram-reaction-negative rods. Nearly all of the cells were non-motile and non-flagellated during the late exponential to early stationary phase of growth, while a few of the cells exhibited motility with monotrichous flagellation. The two isolates required NaCl for growth and grew optimally at about 30 C, 2–3 % NaCl and pH 7–8. They grew aerobically and could achieve anaerobic growth by fermenting D-glucose or other carbohydrates with production of acids and the gases, including CO2 and H2. Ubiquinone Q-8 was the only respiratory quinone. Cellular fatty acids were predominated by C16: 0, C18: 1!7c and C16: 1!7c. The major polar lipid was phosphatidylethanolamine. Strains DM1 and DM2T had DNA G+C contents of 52.0 and 51.8 mol%, respectively, as determined by HPLC analysis. Phylogenetic analyses based on 16S rRNA gene sequences clearly indicated that the two isolates formed a distinct genus-level lineage in the family Aeromonadaceae of the class Gammaproteobacteria and was an outgroup with respect to a stable supragenic clade comprising species of the genera Oceanimonas, Oceanisphaera and Zobellella. The phylogenetic data and those from chemotaxonomic, physiological and morphological characterizations support the establishment of a novel species and genus inside the family Aeromonadaceae, for which the name Dongshaea marina gen. nov., sp. nov. is proposed. The type strain is DM2T (=BCRC 81069T =JCM 32096T).

Original languageEnglish
Article number3080
Pages (from-to)3318-3325
Number of pages8
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume69
Issue number11
DOIs
Publication statusPublished - 2019 Jan 1

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

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    Huang, S. P., Chen, T. Y., Chen, J. S., Wang, L. T., Huang, L., Lin, S. T., Wei, C. L., Lin, S., Wang, P. L., Chen, Y. M., & Shieh, W. Y. (2019). Dongshaea marina gen. Nov., sp. nov., a facultatively anaerobic marine bacterium that ferments glucose with gas production. International Journal of Systematic and Evolutionary Microbiology, 69(11), 3318-3325. [3080]. https://doi.org/10.1099/ijsem.0.003080