TY - JOUR
T1 - Dongshaea marina gen. Nov., sp. nov., a facultatively anaerobic marine bacterium that ferments glucose with gas production
AU - Huang, Ssu Po
AU - Chen, Tzu Yin
AU - Chen, Jwo Sheng
AU - Wang, Li Ting
AU - Huang, Lina
AU - Lin, Shih Ting
AU - Wei, Chih Lin
AU - Lin, Saulwood
AU - Wang, Pei Ling
AU - Chen, Yi Min
AU - Shieh, Wung Yang
N1 - Funding Information:
This study was supported by the Ministry of Science and Technology, Taiwan (grants 102-2621-B-002-006-MY3 and 105-2311-B-002-017).
Funding Information:
Author affiliations: 1Institute of Oceanography, National Taiwan University, Taipei 10617, Taiwan, ROC; 2College of Health Care, China Medical University, No. 91, Shyue-Shyh Rd, Taichung, Taiwan, ROC; 3Bioresource Collection and Research Center, Food Industry Research and Development Institute, Hsinchu 30099, Taiwan, ROC; 4Department of Biotechnology and Bioindustry Sciences, National Cheng Kung University, University Road, Tainan City 701, Taiwan, ROC. *Correspondence: Wung Yang Shieh, [email protected] Keywords: Dongshaea marina; Oceanimonas; Oceanisphaera; Zobellella; Aeromonadaceae; Gammaproteobacteria. Abbreviations: ERIC-PCR, enterobacterial repetitive intergenic consensus-PCR; RAPD-PCR, random amplified polymorphic DNA-PCR; FAMEs, the fatty acid methyl esters. †These authors contributed equally to this work. The GenBank accession numbers for the 16S rRNA gene sequence of strains DM1 and DM2T are MG232374 and MG232375, respectively. The accession numbers for the whole genome sequence of strain DM2T are CP028897 and CP028898. Three supplementary tables and three supplementary figures are available with the online version of this article.
Publisher Copyright:
© 2019, Microbiology Society. All rights reserved.
PY - 2019
Y1 - 2019
N2 - Two isolates of heterotrophic, facultatively anaerobic, marine bacteria, designated DM1 and DM2T, were recovered from a lagoon sediment sample of Dongsha Island, Taiwan. Cells were Gram-reaction-negative rods. Nearly all of the cells were non-motile and non-flagellated during the late exponential to early stationary phase of growth, while a few of the cells exhibited motility with monotrichous flagellation. The two isolates required NaCl for growth and grew optimally at about 30 C, 2–3 % NaCl and pH 7–8. They grew aerobically and could achieve anaerobic growth by fermenting D-glucose or other carbohydrates with production of acids and the gases, including CO2 and H2. Ubiquinone Q-8 was the only respiratory quinone. Cellular fatty acids were predominated by C16: 0, C18: 1!7c and C16: 1!7c. The major polar lipid was phosphatidylethanolamine. Strains DM1 and DM2T had DNA G+C contents of 52.0 and 51.8 mol%, respectively, as determined by HPLC analysis. Phylogenetic analyses based on 16S rRNA gene sequences clearly indicated that the two isolates formed a distinct genus-level lineage in the family Aeromonadaceae of the class Gammaproteobacteria and was an outgroup with respect to a stable supragenic clade comprising species of the genera Oceanimonas, Oceanisphaera and Zobellella. The phylogenetic data and those from chemotaxonomic, physiological and morphological characterizations support the establishment of a novel species and genus inside the family Aeromonadaceae, for which the name Dongshaea marina gen. nov., sp. nov. is proposed. The type strain is DM2T (=BCRC 81069T =JCM 32096T).
AB - Two isolates of heterotrophic, facultatively anaerobic, marine bacteria, designated DM1 and DM2T, were recovered from a lagoon sediment sample of Dongsha Island, Taiwan. Cells were Gram-reaction-negative rods. Nearly all of the cells were non-motile and non-flagellated during the late exponential to early stationary phase of growth, while a few of the cells exhibited motility with monotrichous flagellation. The two isolates required NaCl for growth and grew optimally at about 30 C, 2–3 % NaCl and pH 7–8. They grew aerobically and could achieve anaerobic growth by fermenting D-glucose or other carbohydrates with production of acids and the gases, including CO2 and H2. Ubiquinone Q-8 was the only respiratory quinone. Cellular fatty acids were predominated by C16: 0, C18: 1!7c and C16: 1!7c. The major polar lipid was phosphatidylethanolamine. Strains DM1 and DM2T had DNA G+C contents of 52.0 and 51.8 mol%, respectively, as determined by HPLC analysis. Phylogenetic analyses based on 16S rRNA gene sequences clearly indicated that the two isolates formed a distinct genus-level lineage in the family Aeromonadaceae of the class Gammaproteobacteria and was an outgroup with respect to a stable supragenic clade comprising species of the genera Oceanimonas, Oceanisphaera and Zobellella. The phylogenetic data and those from chemotaxonomic, physiological and morphological characterizations support the establishment of a novel species and genus inside the family Aeromonadaceae, for which the name Dongshaea marina gen. nov., sp. nov. is proposed. The type strain is DM2T (=BCRC 81069T =JCM 32096T).
UR - https://www.scopus.com/pages/publications/85074408301
UR - https://www.scopus.com/pages/publications/85074408301#tab=citedBy
U2 - 10.1099/ijsem.0.003080
DO - 10.1099/ijsem.0.003080
M3 - Article
C2 - 30422104
AN - SCOPUS:85074408301
SN - 1466-5026
VL - 69
SP - 3318
EP - 3325
JO - International Journal of Systematic and Evolutionary Microbiology
JF - International Journal of Systematic and Evolutionary Microbiology
IS - 11
M1 - 3080
ER -