Double-negative (CD4-CD8-) TCRαβ+ cells in patients with systemic lupus erythematosus

Ming-Fei Liu, Jiann Shyong Li, Tien Hsinng Weng, Huan Yao Lei

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4 Citations (Scopus)

Abstract

Massive accumulation of CD4-CD8-TCRαβ+ cells in secondary lymphoid organs is characteristic of lupus-prone MRL/or mice. However, the role of these double negative T cells (DNT) in human lupus patients receive only limited attention. Herein, we investigate the frequency of DNT in the peripheral blood mononuclear cells for forty seven Chinese patients with systemic lupus erythematosus (SLE) and forty four normal individuals. DNT were measured with dual-fluorescence flow cytometry. The results showed that DNT only constituted a very minor subset of lymphocytes both in patients and normals, it normally did not exceed 2% of the lymphocyte population. Compared with normal subjects, patients with SLE had slightly increased levels of DNT within the total lymphocyte population (0.66 ± 0.45% vs 0.51 ± 0.33%) or within TCRαβ+ population (1.14 ± 0.88% vs 0.88 ± 0.54%). The difference, however, did not reach statistical significance. The levels of DNT correlated neither with the titers of anti-DNA antibodies in sera nor with the presence of active and severe lupus nephritis in SLE patients. Longitudinal follow-up of six patients at the stages of active and inactive nephritis revealed similar levels of DNT in the same individual. The preliminary results suggest that circulating DNT do not appear to play a critical role in Chinese patients with SLE.

Original languageEnglish
Pages (from-to)130-134
Number of pages5
JournalScandinavian Journal of Rheumatology
Volume27
Issue number2
DOIs
Publication statusPublished - 1998 Apr 28

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Rheumatology
  • Immunology

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