Drosophila CTP synthase regulates collective cell migration by controlling the polarized endocytic cycle

Pei Yu Wang, Archan Chakraborty, Hsin Ju Ma, Jhen Wei Wu, Anna C.C. Jang, Wei Cheng Lin, Hai Wei Pi, Chau Ting Yeh, Mei Ling Cheng, Jau Song Yu, Li Mei Pai

Research output: Contribution to journalArticlepeer-review

Abstract

Phosphatidylinositol (PI) 4,5-bisphosphate (PIP2) is involved in many biological functions. However, the mechanisms of PIP2 in collective cell migration remain elusive. This study highlights the regulatory role of cytidine triphosphate synthase (CTPsyn) in collective border cell migration through regulating the asymmetrical distribution of PIP2. We demonstrated that border cell clusters containing mutant CTPsyn cells suppressed migration. CTPsyn was co-enriched with Actin at the leading edge of the Drosophila border cell cluster where PIP2 was enriched, and this enrichment depended on the CTPsyn activity. Genetic interactions of border cell migration were found between CTPsyn mutant and genes in PI biosynthesis. The CTPsyn reduction resulted in loss of the asymmetric activity of endocytosis recycling. Also, genetic interactions were revealed between components of the exocyst complex and CTPsyn mutant, indicating that CTPsyn activity regulates the PIP2-related asymmetrical exocytosis activity. Furthermore, CTPsyn activity is essential for RTK-polarized distribution in the border cell cluster. We propose a model in which CTPsyn activity is required for the asymmetrical generation of PIP2 to enrich RTK signaling through endocytic recycling in collective cell migration.

Original languageEnglish
JournalJournal of Embryology and Experimental Morphology
Volume149
Issue number16
DOIs
Publication statusPublished - 2022 Aug 15

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Developmental Biology

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