Dual Methylated BRCA1/BRCA2 Detection on an Aptamer-Based Integrated Microfluidic System

Chih Hung Wang; Keng Fu Hsu; Gwo Bin Lee

Dual Methylated BRCA1/BRCA2 Detection on an Aptamer-Based Integrated Microfluidic System

Chih Hung Wang
, Keng Fu Hsu
, Gwo Bin Lee

Department of Obstetrics Gynecology

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

A new screening strategy has been developed to select a new aptamer suitably used in a clinical environment by using an in vivo-like systematic evolution of ligands by exponential enrichment (SELEX) following by conventional position and negative selection processes. One novel methylation-specific aptamer with high affinity and high specificity was successfully selected such that it could capture methylated tumor circulating cell-free DNA (cfDNA) from plasma of ovarian cancer (OvCa) patients. The aptamer was conjugated onto magnetic beads and applied in an integrated microfluidic chip for detection and quantification of two methylated tumor suppressor genes, BRCA1/BRCA2, from cfDNA in plasma or blood.

Original language	English
Title of host publication	2023 22nd International Conference on Solid-State Sensors, Actuators and Microsystems, Transducers 2023
Publisher	Institute of Electrical and Electronics Engineers Inc.
Pages	160-163
Number of pages	4
ISBN (Electronic)	9784886864352
Publication status	Published - 2023
Event	22nd International Conference on Solid-State Sensors, Actuators and Microsystems, Transducers 2023 - Kyoto, Japan Duration: 2023 Jun 25 → 2023 Jun 29

Publication series

Name	2023 22nd International Conference on Solid-State Sensors, Actuators and Microsystems, Transducers 2023

Conference

Conference	22nd International Conference on Solid-State Sensors, Actuators and Microsystems, Transducers 2023
Country/Territory	Japan
City	Kyoto
Period	23-06-25 → 23-06-29

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

All Science Journal Classification (ASJC) codes

Computer Science Applications
Electrical and Electronic Engineering
Control and Optimization
Instrumentation

Cite this

Wang, C. H., Hsu, K. F., & Lee, G. B. (2023). Dual Methylated BRCA1/BRCA2 Detection on an Aptamer-Based Integrated Microfluidic System. In 2023 22nd International Conference on Solid-State Sensors, Actuators and Microsystems, Transducers 2023 (pp. 160-163). (2023 22nd International Conference on Solid-State Sensors, Actuators and Microsystems, Transducers 2023). Institute of Electrical and Electronics Engineers Inc..

Wang, Chih Hung ; Hsu, Keng Fu ; Lee, Gwo Bin. / Dual Methylated BRCA1/BRCA2 Detection on an Aptamer-Based Integrated Microfluidic System. 2023 22nd International Conference on Solid-State Sensors, Actuators and Microsystems, Transducers 2023. Institute of Electrical and Electronics Engineers Inc., 2023. pp. 160-163 (2023 22nd International Conference on Solid-State Sensors, Actuators and Microsystems, Transducers 2023).

@inproceedings{62188c59233f411b9fc9267964aca3df,

title = "Dual Methylated BRCA1/BRCA2 Detection on an Aptamer-Based Integrated Microfluidic System",

abstract = "A new screening strategy has been developed to select a new aptamer suitably used in a clinical environment by using an in vivo-like systematic evolution of ligands by exponential enrichment (SELEX) following by conventional position and negative selection processes. One novel methylation-specific aptamer with high affinity and high specificity was successfully selected such that it could capture methylated tumor circulating cell-free DNA (cfDNA) from plasma of ovarian cancer (OvCa) patients. The aptamer was conjugated onto magnetic beads and applied in an integrated microfluidic chip for detection and quantification of two methylated tumor suppressor genes, BRCA1/BRCA2, from cfDNA in plasma or blood.",

author = "Wang, \{Chih Hung\} and Hsu, \{Keng Fu\} and Lee, \{Gwo Bin\}",

note = "Publisher Copyright: {\textcopyright} 2023 IEEJ.; 22nd International Conference on Solid-State Sensors, Actuators and Microsystems, Transducers 2023 ; Conference date: 25-06-2023 Through 29-06-2023",

year = "2023",

language = "English",

series = "2023 22nd International Conference on Solid-State Sensors, Actuators and Microsystems, Transducers 2023",

publisher = "Institute of Electrical and Electronics Engineers Inc.",

pages = "160--163",

booktitle = "2023 22nd International Conference on Solid-State Sensors, Actuators and Microsystems, Transducers 2023",

address = "United States",

}

Wang, CH, Hsu, KF & Lee, GB 2023, Dual Methylated BRCA1/BRCA2 Detection on an Aptamer-Based Integrated Microfluidic System. in 2023 22nd International Conference on Solid-State Sensors, Actuators and Microsystems, Transducers 2023. 2023 22nd International Conference on Solid-State Sensors, Actuators and Microsystems, Transducers 2023, Institute of Electrical and Electronics Engineers Inc., pp. 160-163, 22nd International Conference on Solid-State Sensors, Actuators and Microsystems, Transducers 2023, Kyoto, Japan, 23-06-25.

Dual Methylated BRCA1/BRCA2 Detection on an Aptamer-Based Integrated Microfluidic System. / Wang, Chih Hung; Hsu, Keng Fu; Lee, Gwo Bin.
2023 22nd International Conference on Solid-State Sensors, Actuators and Microsystems, Transducers 2023. Institute of Electrical and Electronics Engineers Inc., 2023. p. 160-163 (2023 22nd International Conference on Solid-State Sensors, Actuators and Microsystems, Transducers 2023).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

TY - GEN

T1 - Dual Methylated BRCA1/BRCA2 Detection on an Aptamer-Based Integrated Microfluidic System

AU - Wang, Chih Hung

AU - Hsu, Keng Fu

AU - Lee, Gwo Bin

PY - 2023

Y1 - 2023

N2 - A new screening strategy has been developed to select a new aptamer suitably used in a clinical environment by using an in vivo-like systematic evolution of ligands by exponential enrichment (SELEX) following by conventional position and negative selection processes. One novel methylation-specific aptamer with high affinity and high specificity was successfully selected such that it could capture methylated tumor circulating cell-free DNA (cfDNA) from plasma of ovarian cancer (OvCa) patients. The aptamer was conjugated onto magnetic beads and applied in an integrated microfluidic chip for detection and quantification of two methylated tumor suppressor genes, BRCA1/BRCA2, from cfDNA in plasma or blood.

AB - A new screening strategy has been developed to select a new aptamer suitably used in a clinical environment by using an in vivo-like systematic evolution of ligands by exponential enrichment (SELEX) following by conventional position and negative selection processes. One novel methylation-specific aptamer with high affinity and high specificity was successfully selected such that it could capture methylated tumor circulating cell-free DNA (cfDNA) from plasma of ovarian cancer (OvCa) patients. The aptamer was conjugated onto magnetic beads and applied in an integrated microfluidic chip for detection and quantification of two methylated tumor suppressor genes, BRCA1/BRCA2, from cfDNA in plasma or blood.

UR - https://www.scopus.com/pages/publications/85193517519

UR - https://www.scopus.com/pages/publications/85193517519#tab=citedBy

M3 - Conference contribution

AN - SCOPUS:85193517519

T3 - 2023 22nd International Conference on Solid-State Sensors, Actuators and Microsystems, Transducers 2023

SP - 160

EP - 163

BT - 2023 22nd International Conference on Solid-State Sensors, Actuators and Microsystems, Transducers 2023

PB - Institute of Electrical and Electronics Engineers Inc.

T2 - 22nd International Conference on Solid-State Sensors, Actuators and Microsystems, Transducers 2023

Y2 - 25 June 2023 through 29 June 2023

ER -

Wang CH, Hsu KF, Lee GB. Dual Methylated BRCA1/BRCA2 Detection on an Aptamer-Based Integrated Microfluidic System. In 2023 22nd International Conference on Solid-State Sensors, Actuators and Microsystems, Transducers 2023. Institute of Electrical and Electronics Engineers Inc. 2023. p. 160-163. (2023 22nd International Conference on Solid-State Sensors, Actuators and Microsystems, Transducers 2023).

Dual Methylated BRCA1/BRCA2 Detection on an Aptamer-Based Integrated Microfluidic System

Abstract

Publication series

Conference

UN SDGs

All Science Journal Classification (ASJC) codes

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