Dual-specificity phosphatase 6 deficiency regulates gut microbiome and transcriptome response against diet-induced obesity in mice

Jhen-Wei Ruan, Sarah Statt, Chih Ting Huang, Yi Ting Tsai, Cheng Chin Kuo, Hong Lin Chan, Yu Chieh Liao, Tse Hua Tan, Cheng Yuan Kao

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The gut microbiota plays profound roles in host metabolism and the inflammatory response associated with the development of obesity. Dusp6-deficient mice have been shown to be resistant to diet-induced obesity, but the mechanism behind this remains unclear. 16S ribosomal RNA gene analysis demonstrated that dusp6-deficient mice harbour unique gut microbiota with resistance to diet-induced-obesity-mediated alteration of the gut microbiome. Using a germ-free mouse model, we found that faecal/gut microbiota derived from dusp6-deficient mice significantly increased energy expenditure and reduced weight gain in recipient wild-type mice fed on a high-fat diet. On analysis of the intestinal transcriptome of dusp6-deficient mice, we found that dusp6 deficiency mainly induced biological processes involved in metabolism and the extracellular matrix, particularly the peroxisome proliferator-activated receptor gamma (Pparγ) pathway and tight-junction genes. Furthermore, dusp6-deficient mice have a high-fat-diet-specific transcriptomic response to reverse the expression of genes associated with intestinal barrier functions and mucosal immunity involved in microbiome homeostasis. This study demonstrates that dusp6 deficiency is a strong genetic factor shaping gut microbiota, and that it confers obesity protection by ameliorating the gut microbiota response to diet-mediated stress.

Original languageEnglish
Article number16220
JournalNature Microbiology
Volume2
DOIs
Publication statusPublished - 2016 Nov 28

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Dual Specificity Phosphatase 6
Transcriptome
Obesity
Diet
High Fat Diet
16S Ribosomal RNA
Biological Phenomena
Mucosal Immunity
Tight Junctions
PPAR gamma
Microbiota
Gene Expression Profiling
Gastrointestinal Microbiome
rRNA Genes
Energy Metabolism
Weight Gain
Extracellular Matrix
Homeostasis
Gene Expression

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Applied Microbiology and Biotechnology
  • Genetics
  • Microbiology (medical)
  • Cell Biology

Cite this

Ruan, Jhen-Wei ; Statt, Sarah ; Huang, Chih Ting ; Tsai, Yi Ting ; Kuo, Cheng Chin ; Chan, Hong Lin ; Liao, Yu Chieh ; Tan, Tse Hua ; Kao, Cheng Yuan. / Dual-specificity phosphatase 6 deficiency regulates gut microbiome and transcriptome response against diet-induced obesity in mice. In: Nature Microbiology. 2016 ; Vol. 2.
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Dual-specificity phosphatase 6 deficiency regulates gut microbiome and transcriptome response against diet-induced obesity in mice. / Ruan, Jhen-Wei; Statt, Sarah; Huang, Chih Ting; Tsai, Yi Ting; Kuo, Cheng Chin; Chan, Hong Lin; Liao, Yu Chieh; Tan, Tse Hua; Kao, Cheng Yuan.

In: Nature Microbiology, Vol. 2, 16220, 28.11.2016.

Research output: Contribution to journalArticle

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