Dynamic metabolic profiling together with transcription analysis reveals salinity-induced starch-To-lipid biosynthesis in alga Chlamydomonas sp. JSC4

Shih Hsin Ho, Akihito Nakanishi, Yuichi Kato, Hiroaki Yamasaki, Jo Shu Chang, Naomi Misawa, Yuu Hirose, Jun Minagawa, Tomohisa Hasunuma, Akihiko Kondo

Research output: Contribution to journalArticlepeer-review

56 Citations (Scopus)

Abstract

Biodiesel production using microalgae would play a pivotal role in satisfying future global energy demands. Understanding of lipid metabolism in microalgae is important to isolate oleaginous strain capable of overproducing lipids. It has been reported that reducing starch biosynthesis can enhance lipid accumulation. However, the metabolic mechanism controlling carbon partitioning from starch to lipids in microalgae remains unclear, thus complicating the genetic engineering of algal strains. We here used "dynamic" metabolic profiling and essential transcription analysis of the oleaginous green alga Chlamydomonas sp. JSC4 for the first time to demonstrate the switching mechanisms from starch to lipid synthesis using salinity as a regulator, and identified the metabolic rate-limiting step for enhancing lipid accumulation (e.g., pyruvate-To-Acetyl-CoA). These results, showing salinity-induced starch-To-lipid biosynthesis, will help increase our understanding of dynamic carbon partitioning in oleaginous microalgae. Moreover, we successfully determined the changes of several key lipid-synthesis-related genes (e.g., acetyl-CoA carboxylase, pyruvate decarboxylase, acetaldehyde dehydrogenase, acetyl-CoA synthetase and pyruvate ferredoxin oxidoreductase) and starch-degradation related genes (e.g., starch phosphorylases), which could provide a breakthrough in the marine microalgal production of biodiesel.

Original languageEnglish
Article number45471
JournalScientific reports
Volume7
DOIs
Publication statusPublished - 2017 Apr 4

All Science Journal Classification (ASJC) codes

  • General

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