Dynamic synergistic effect on Trichoderma reesei cellulases by novel β-glucosidases from Taiwanese fungi

I. Son Ng, Shau Wei Tsai, Yu Ming Ju, Su May Yu, Tuan hua David Ho

Research output: Contribution to journalArticlepeer-review

39 Citations (Scopus)

Abstract

Dynamic synergistic effects in cellulosic bioconversion have been revealed between Trichoderma reesei cellulases and β-glucosidases (BGLs) from six Taiwanese fungi. A high level of synergy (8.9-fold) was observed with the addition of Chaetomella raphigera BGL to T. reesei cellulases. In addition, the C. raphigera BGL possessed the highest activity (Vmax/Km=46.6U/mgmM) and lowest glucose inhibition (Ki=4.6mM) with the substrate 4-nitrophenyl β-d-glucopyranoside. For the natural cellobiose substrate, however, the previously isolated Aspergillus niger BGL Novo-188 had the highest Vmax/Km (0.72U/mgmM) and lowest Ki (59.5mM). The demonstrated dynamic synergistic effects between some BGLs and the T. reesei cellulase system suggest that BGLs not only prevent the inhibition by cellobiose, but also enhance activities of endo- and exo-cellulases in cellulosic bioconversion. Comparisons of kinetic parameters and synergism analyses between BGLs and T. reesei cellulases can be used for further optimization of the cellulosic bioconversion process.

Original languageEnglish
Pages (from-to)6073-6081
Number of pages9
JournalBioresource technology
Volume102
Issue number10
DOIs
Publication statusPublished - 2011 May 1

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Environmental Engineering
  • Renewable Energy, Sustainability and the Environment
  • Waste Management and Disposal

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