Dysfunction of the mitochondrial respiratory chain in the rostral ventrolateral medulla during experimental endotoxemia in the rat

Yao Chung Chuang, Jin Lian Tsai, Ya-Wen Zhang, Julie Y.H. Chan, Chai Wei Liou, Samuel H.H. Chan

Research output: Contribution to journalArticle

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Abstract

We investigated the functional changes in the mitochondrial respiratory chain at the rostral ventrolateral medulla (RVLM), the medullary origin of sympathetic vasomotor tone, in an experimental model of endotoxemia that mimics systemic inflammatory response syndrome. In Sprague-Dawley rats maintained under propofol anesthesia, intravenous administration of Escherichia coli lipopolysaccharide (LPS; 30 mg/kg) induced a reduction (Phase I), followed by an augmentation (Phase II) and a secondary decrease (Phase III) in the power density of vasomotor components (0-0.8 Hz) in systemic arterial pressure signals. LPS also elicited progressive hypotension, and death ensued within 4 h. Enzyme assay revealed significant depression of the activity of nicotinamide adenine dinucleotide cytochrome c reductase (Complexes I +III) and cytochromecoxidase (Complex IV) in the RVLM during all three phases of endotoxemia. On the other hand, the activity of succinate cytochromec reductase (Complexes II +III) remained unaltered. We conclude that selective dysfunction of respiratory enzyme Complexes I and IV in the mitochondrial respiratory chain at the RVLM, whose neuronal activity is intimately related to the death process, is closely associated with fatal endotoxemia in the rat.

Original languageEnglish
Pages (from-to)542-548
Number of pages7
JournalJournal of biomedical science
Volume9
Issue number5-6
DOIs
Publication statusPublished - 2002 Jan 1

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Endotoxemia
Electron Transport
Rats
Oxidoreductases
Succinic Acid
Propofol
Enzymes
Cytochromes c
NAD
Cytochrome Reductases
Escherichia coli
Electron Transport Complex I
Lipopolysaccharides
Systemic Inflammatory Response Syndrome
Assays
Enzyme Assays
Intravenous Administration
Hypotension
Sprague Dawley Rats
Arterial Pressure

All Science Journal Classification (ASJC) codes

  • Endocrinology, Diabetes and Metabolism
  • Molecular Biology
  • Clinical Biochemistry
  • Cell Biology
  • Biochemistry, medical
  • Pharmacology (medical)

Cite this

Chuang, Yao Chung ; Tsai, Jin Lian ; Zhang, Ya-Wen ; Chan, Julie Y.H. ; Liou, Chai Wei ; Chan, Samuel H.H. / Dysfunction of the mitochondrial respiratory chain in the rostral ventrolateral medulla during experimental endotoxemia in the rat. In: Journal of biomedical science. 2002 ; Vol. 9, No. 5-6. pp. 542-548.
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Dysfunction of the mitochondrial respiratory chain in the rostral ventrolateral medulla during experimental endotoxemia in the rat. / Chuang, Yao Chung; Tsai, Jin Lian; Zhang, Ya-Wen; Chan, Julie Y.H.; Liou, Chai Wei; Chan, Samuel H.H.

In: Journal of biomedical science, Vol. 9, No. 5-6, 01.01.2002, p. 542-548.

Research output: Contribution to journalArticle

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