Early signalling pathways in rice roots under vanadate stress

Chung Wen Lin, Chung Yi Lin, Ching Chun Chang, Ruey Hua Lee, Tsung Mu Tsai, Po Yu Chen, Wen Chang Chi, Hao Jen Huang

Research output: Contribution to journalArticlepeer-review

22 Citations (Scopus)

Abstract

Vanadate is beneficial to plant growth at low concentration. However, plant exposure to high concentrations of vanadate has been shown to arrest cell growth and lead to cell death. We are interested in understanding the signalling pathways of rice roots in response to vanadate stress. In this study, we demonstrated that vanadate induced rice root cell death and suppressed root growth. In addition, we found that vanadate induced ROS accumulation, increased lipid peroxidation and elicited a remarkable increase of MAPKs and CDPKs activities in rice roots. In contrast, pre-treatment of rice roots with ROS scavenger (sodium benzoate), serine/threonine protein phosphatase inhibitor (endothall), and CDPK antagonist (W7), reduced the vanadate-induced MAPKs activation. Furthermore, the expression of a MAPK gene (OsMPK3) and four tyrosine phosphatase genes (OsDSP3, OsDSP5, OsDSP6, and OsDSP10) were regulated by vanadate in rice roots. Collectively, these results strongly suggest that ROS, protein phosphatase, and CDPK may function in the vanadate-triggered MAPK signalling pathway cause cell death and retarded growth in rice roots.

Original languageEnglish
Pages (from-to)369-376
Number of pages8
JournalPlant Physiology and Biochemistry
Volume47
Issue number5
DOIs
Publication statusPublished - 2009 May 1

All Science Journal Classification (ASJC) codes

  • Physiology
  • Genetics
  • Plant Science

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