Early signalling pathways in rice roots under vanadate stress

Chung Wen Lin, Chung Yi Lin, Ching-Chun Chang, Ruey-Hua Lee, Tsung Mu Tsai, Po Yu Chen, Wen Chang Chi, Hao-Jen Huang

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Vanadate is beneficial to plant growth at low concentration. However, plant exposure to high concentrations of vanadate has been shown to arrest cell growth and lead to cell death. We are interested in understanding the signalling pathways of rice roots in response to vanadate stress. In this study, we demonstrated that vanadate induced rice root cell death and suppressed root growth. In addition, we found that vanadate induced ROS accumulation, increased lipid peroxidation and elicited a remarkable increase of MAPKs and CDPKs activities in rice roots. In contrast, pre-treatment of rice roots with ROS scavenger (sodium benzoate), serine/threonine protein phosphatase inhibitor (endothall), and CDPK antagonist (W7), reduced the vanadate-induced MAPKs activation. Furthermore, the expression of a MAPK gene (OsMPK3) and four tyrosine phosphatase genes (OsDSP3, OsDSP5, OsDSP6, and OsDSP10) were regulated by vanadate in rice roots. Collectively, these results strongly suggest that ROS, protein phosphatase, and CDPK may function in the vanadate-triggered MAPK signalling pathway cause cell death and retarded growth in rice roots.

Original languageEnglish
Pages (from-to)369-376
Number of pages8
JournalPlant Physiology and Biochemistry
Volume47
Issue number5
DOIs
Publication statusPublished - 2009 May 1

Fingerprint

Vanadates
rice
cell death
Cell Death
Phosphoprotein Phosphatases
Growth
sodium benzoate
phosphoprotein phosphatase
Sodium Benzoate
tyrosine
Oryza
antagonists
cell growth
stress response
root growth
lipid peroxidation
genes
pretreatment
Phosphoric Monoester Hydrolases
plant growth

All Science Journal Classification (ASJC) codes

  • Physiology
  • Genetics
  • Plant Science

Cite this

Lin, Chung Wen ; Lin, Chung Yi ; Chang, Ching-Chun ; Lee, Ruey-Hua ; Tsai, Tsung Mu ; Chen, Po Yu ; Chi, Wen Chang ; Huang, Hao-Jen. / Early signalling pathways in rice roots under vanadate stress. In: Plant Physiology and Biochemistry. 2009 ; Vol. 47, No. 5. pp. 369-376.
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Early signalling pathways in rice roots under vanadate stress. / Lin, Chung Wen; Lin, Chung Yi; Chang, Ching-Chun; Lee, Ruey-Hua; Tsai, Tsung Mu; Chen, Po Yu; Chi, Wen Chang; Huang, Hao-Jen.

In: Plant Physiology and Biochemistry, Vol. 47, No. 5, 01.05.2009, p. 369-376.

Research output: Contribution to journalArticle

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