TY - JOUR
T1 - Effect of Cassia fistula Linn. leaf extract on diethylnitrosamine induced hepatic injury in rats
AU - Pradeep, Kannampalli
AU - Mohan, Chandrasekaran Victor Raj
AU - Gobianand, Kuppannan
AU - Karthikeyan, Sivanesan
N1 - Funding Information:
The authors wish to thank the UGC-UWPFE Project (HS-43) for the financial assistance provided for this study.
PY - 2007/4/5
Y1 - 2007/4/5
N2 - The hepatoprotective and antioxidant effect of Cassia fistula Linn. leaf extract on liver injury induced by diethylnitrosamine (DEN) was investigated. Wistar rats weighing 200 ± 10 g were administered a single dose of DEN (200 mg/kg b.w., i.p.) and left for 30 days. For hepatoprotective studies, ethanolic leaf extract (ELE) of C. fistula Linn. (500 mg/kg b.w., p.o.) was administered daily for 30 days. AST, ALT, ALP, LDH, γ-GT and bilirubin were estimated in serum and liver tissue. Lipid peroxidation (LPO), SOD and CAT were also estimated in liver tissue as markers of oxidative stress. DEN induced hepatotoxicity in all the treated animals were evident by elevated serum ALT, AST, ALP and bilirubin levels and a simultaneous fall in their levels in the liver tissue after 30 days. Induction of oxidative stress in the liver was evidenced by increased LPO and fall in the activities of SOD and CAT. ELE administration for 30 days prevented the DEN induced hepatic injury and oxidative stress. In conclusion, it was observed that ELE of C. fistula Linn. protects the liver against DEN induced hepatic injury in rats.
AB - The hepatoprotective and antioxidant effect of Cassia fistula Linn. leaf extract on liver injury induced by diethylnitrosamine (DEN) was investigated. Wistar rats weighing 200 ± 10 g were administered a single dose of DEN (200 mg/kg b.w., i.p.) and left for 30 days. For hepatoprotective studies, ethanolic leaf extract (ELE) of C. fistula Linn. (500 mg/kg b.w., p.o.) was administered daily for 30 days. AST, ALT, ALP, LDH, γ-GT and bilirubin were estimated in serum and liver tissue. Lipid peroxidation (LPO), SOD and CAT were also estimated in liver tissue as markers of oxidative stress. DEN induced hepatotoxicity in all the treated animals were evident by elevated serum ALT, AST, ALP and bilirubin levels and a simultaneous fall in their levels in the liver tissue after 30 days. Induction of oxidative stress in the liver was evidenced by increased LPO and fall in the activities of SOD and CAT. ELE administration for 30 days prevented the DEN induced hepatic injury and oxidative stress. In conclusion, it was observed that ELE of C. fistula Linn. protects the liver against DEN induced hepatic injury in rats.
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U2 - 10.1016/j.cbi.2006.12.011
DO - 10.1016/j.cbi.2006.12.011
M3 - Article
C2 - 17289008
AN - SCOPUS:33947095422
SN - 0009-2797
VL - 167
SP - 12
EP - 18
JO - Chemico-Biological Interactions
JF - Chemico-Biological Interactions
IS - 1
ER -