Effect of collagen digestion on the passive elastic properties of diaphragm muscle in rat

Justin Rowe, Qingshan Chen, Zachary J. Domire, Matthew B. McCullough, Gary Sieck, Wen Zhi Zhan, Kai Nan An

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)

Abstract

Effects of collagen digestion have been defined up to the fibril level. However, the question remains as to whether the alteration of skeletal muscle extracellular matrix (ECM) affects a muscle's passive elastic response. Various elastography methods have been applied as tools for evaluating the mechanical properties and ECM content of skeletal muscle. In an effort to develop an ECM altered skeletal muscle model, this study determined the effect of collagen digestion on the passive elastic properties of skeletal muscle. Passive mechanical properties of rat diaphragms were evaluated in various degrees of collagen digestion. Between cyclic loading tests, muscle strips were immersed in various concentrations of clostridium histolyticum derived bacterial collagenase. All samples were later viewed via light microscopy. Cyclic testing revealed linear relationships between passive muscle stiffness and digestion time at multiple concentrations. These results demonstrate that collagenase digestion of the ECM in skeletal muscle could be used as a simple and reliable model of mechanically altered in vitro tissue samples.

Original languageEnglish
Pages (from-to)90-94
Number of pages5
JournalMedical Engineering and Physics
Volume32
Issue number1
DOIs
Publication statusPublished - 2010 Jan

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biomedical Engineering

Fingerprint

Dive into the research topics of 'Effect of collagen digestion on the passive elastic properties of diaphragm muscle in rat'. Together they form a unique fingerprint.

Cite this