Effect of CO₂ laser on healing of cultured meniscus

Background and Objective: A new method to improve cartilage repair is clinically important. The enhancement of meniscal healing by low power CO₂ laser was investigated in an organ culture system. Study Design/Materials and Methods: A longitudinal or a radial defect was made in the avascular zone of rabbit menisci. Irradiation by CO₂ laser with 1 W (energy density 50 J/cm²) and 2 W (energy density 100 J/cm²) was used. Results: Histologic and scanning electron microscopic evaluations revealed that both energy densities of laser irradiation and the type of and the site of meniscal defect can influence the course and the outcome of meniscal healing. A marked increase in fibrochondrocytic proliferation and regeneration of collagen fibers were demonstrated in the meniscal defects irradiated by 100 J of CO₂ laser energy. Conclusions: The healing of meniscal defects could be promoted by low power CO₂ laser irradiation.