Effect of dietary β-1,3-glucan on resistance to white spot syndrome virus (WSSV) in postlarval and juvenile Penaeus monodon

Cheng Fang Chang, Mao Sen Su, Houng Yung Chen, Chu Fang Lo, Guang Hsiung Kou, I. Chiu Liao

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87 Citations (Scopus)

Abstract

This study evaluated the effectiveness of dietary incorporation of β-1,3-glucan from Schizophyllum commune in enhancing the resistance of postlarval (PL15) or juvenile (5.5 ± 0.5 g) Penaeus monodon to white spot syndrome virus (WSSV). The shrimp were fed experimental diets with (2 g kg-1 diet) or without β-1,3-glucan for 15 d (postlarvae) or 10 or 20 d (juveniles). Following these dietary treatments, the postlarvae were challenged by immersion and the juveniles by injection using WSSV solution. After challenge with WSSV, initial mortality was significantly (p < 0.005) lower in all the glucan-fed groups than in the respective non-glucan control groups. In the juvenile groups, glucan supplementation for 20 d rather than 10 d significantly (p < 0.005) enhanced survival. After the WSSV challenge, none of the control diet postlarvae or juveniles survived beyond 4 d and none of the 10 d glucan-fed juveniles survived beyond 6 d. By contrast, some of the glucan-treated postlarvae (12.2%) and some of the 20 d glucan-treated juveniles (20%) were still alive at Day 6. These surviving individuals were reared onwards up to 120 d post-challenge, at which time overall survivals were 5.5% (postlarvae) and 13.3% (juveniles) and body weights were 25 to 30 g (postlarvae) and 30 to 40 g (juveniles). Half of the surviving postlarvae and 58% of the surviving juveniles were 2-step WSSV polymerase chain reaction (PCR) negative 6 d after challenge. All the surviving shrimp were 2-step WSSV PCR negative 120 d after challenge.

Original languageEnglish
Pages (from-to)163-168
Number of pages6
JournalDiseases of Aquatic Organisms
Volume36
Issue number3
DOIs
Publication statusPublished - 1999 May 31

All Science Journal Classification (ASJC) codes

  • Ecology, Evolution, Behavior and Systematics
  • Aquatic Science

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