Amyloid-β (Aβ) was showed to be able to induce the apoptosis of neuronal cells leading to the occurrence of Alzheimer's disease (AD). As those cells were stimulated by optical energy, the Aβ induced apoptosis was greatly inhibited. Hence, current study aims to further explore the effect of ultrasonic insonification on the neuronal cells. Experiments on PC-12 cells added with a 20 μM Aβ 25-35 to induce cellular apoptosis were performed. These cells were stimulated by 1MHz ultrasound of a fixed 150 mW/cm2 (SATA) intensity associated respectively with different exposure durations from 1 to 10 minutes and duty cycles from 10 to 100%. The response of cells after acoustic stimulation for 24 and 48 hours was assessed via microscopic morphology and cell death measured by typical MTT assay. Results showed that the cell death was not significantly different corresponding to those cells with or without adding Aβ under ultrasonic stimulation. In accordance with 150mW/cm2 ultrasound exposure of 20% duty cycle for 3 minutes, Aβ induced neuronal cells apoptosis was decreased. This study demonstrated that the effect of diminishing neuronal cells from apoptosis could be regulated by the insonation of appropriate ultrasonic doses.