Effect of forms of collagen linked to polyurethane on endothelial cell growth

P. C. Lee, Lynn L.H. Huang, L. W. Chen, K. H. Hsieh, C. L. Tsai

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24 Citations (Scopus)


Collagen has been widely coated or grafted onto polymer surfaces to improve the biocompatibility of materials. To better support the growth of endothelial cells on polyurethane (PU), collagen was grafted to the carboxyl group enriched PU through 1,2-bis(2,3-epoxypropoxy)ethane linking. Our results demonstrated that collagen in various conditions may result in different forms being grafted to the PU substrate, which subsequently affected the growth of endothelial cells. Collagen predialyzed against physiological phosphate buffered saline (PBS) could be reconstituted into native type fibrils with a bigger diameter at 37°C than could collagen neutralized by titration with NaOH. At low temperature, titrated collagen formed flosslike fibrils packed in a ball with cobblestonelike morphology. The amount of collagen grafted was related to the condition of the collagen used, which in consequence affected the diameter of the collagen fibril formed and the growth of endothelial cells. In conclusion, reconstituted collagen fibrils formed from collagen in PBS at 37°C grafted in the highest amounts to an epoxy-PU substrate and that optimally supported the growth of endothelial cells. Such prepared materials may be potentially good vascular bioprosthetic materials and may provide a wide range of biological applications.

Original languageEnglish
Pages (from-to)645-653
Number of pages9
JournalJournal of Biomedical Materials Research
Issue number4
Publication statusPublished - 1996 Dec 1

All Science Journal Classification (ASJC) codes

  • Biomaterials
  • Biomedical Engineering


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