Effect of low-intensity pulsed ultrasound on the nano-mechanical properties of cholesterol-manipulated cells

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

The mechanical properties of cells have been verified to correlate to human diseases and aging as well as to various cellular physiological processes, including proliferation, migration, and differentiation. It thus is essential to investigate responses of cells corresponding to chemical and physical stimulations for cellular mechanics studies and other applications. Moreover, measurement of the stiffness of an interested cell is of great importance to comprehend the cellular response with respect to a specific biophysical or biochemical alteration. Hence, current study aims to investigate the effect of low-intensity pulsed ultrasound (LIPUS) on nano-mechanical properties of cholesterol-manipulated cells. The NIH-3T3 fibroblastswere firstly incubated in cholesterol depletion solution. The cells were then insonified by a 1 MHz LIPUS for three minutes during the process of cholesterol restoration. In situ observation on the variations of living cells adhered on the glass substrate was monitored continuously. The cellular morphological changes were recorded for 8 hours. The nano-mechanical properties of cells were detected with nano-indentation by measuring the cellular membrane of a single cell. The cell properties seemed to be unchanged when adding with serum. On the other hand, the cholesterol depletion tended to lead a majority of cells to retract and become rounding corresponding to the increase of incubation time. The complications may be alleviated with the insonification of LIPUS. The elastic properties of those cholesterol-manipulated cells following LIPUS insonification tended to be improved. This study demonstrates that the variations of nano-mechanical property of cell membrane during cholesterol restoration and LIPUS insonification may be sensitively detected using the depth-sensing nano-indentation technique. The LIPUS insonification may be beneficial to the restoration of elastic properties of cholesterol-manipulated cells.

Original languageEnglish
Title of host publication2012 IEEE International Ultrasonics Symposium, IUS 2012
Pages600-603
Number of pages4
DOIs
Publication statusPublished - 2012
Event2012 IEEE International Ultrasonics Symposium, IUS 2012 - Dresden, Germany
Duration: 2012 Oct 72012 Oct 10

Other

Other2012 IEEE International Ultrasonics Symposium, IUS 2012
CountryGermany
CityDresden
Period12-10-0712-10-10

Fingerprint

cholesterol
mechanical properties
cells
restoration
nanoindentation
depletion
elastic properties
stimulation
serums
stiffness
membranes

All Science Journal Classification (ASJC) codes

  • Acoustics and Ultrasonics

Cite this

@inproceedings{209de82ae9fb425aa155754a482936f2,
title = "Effect of low-intensity pulsed ultrasound on the nano-mechanical properties of cholesterol-manipulated cells",
abstract = "The mechanical properties of cells have been verified to correlate to human diseases and aging as well as to various cellular physiological processes, including proliferation, migration, and differentiation. It thus is essential to investigate responses of cells corresponding to chemical and physical stimulations for cellular mechanics studies and other applications. Moreover, measurement of the stiffness of an interested cell is of great importance to comprehend the cellular response with respect to a specific biophysical or biochemical alteration. Hence, current study aims to investigate the effect of low-intensity pulsed ultrasound (LIPUS) on nano-mechanical properties of cholesterol-manipulated cells. The NIH-3T3 fibroblastswere firstly incubated in cholesterol depletion solution. The cells were then insonified by a 1 MHz LIPUS for three minutes during the process of cholesterol restoration. In situ observation on the variations of living cells adhered on the glass substrate was monitored continuously. The cellular morphological changes were recorded for 8 hours. The nano-mechanical properties of cells were detected with nano-indentation by measuring the cellular membrane of a single cell. The cell properties seemed to be unchanged when adding with serum. On the other hand, the cholesterol depletion tended to lead a majority of cells to retract and become rounding corresponding to the increase of incubation time. The complications may be alleviated with the insonification of LIPUS. The elastic properties of those cholesterol-manipulated cells following LIPUS insonification tended to be improved. This study demonstrates that the variations of nano-mechanical property of cell membrane during cholesterol restoration and LIPUS insonification may be sensitively detected using the depth-sensing nano-indentation technique. The LIPUS insonification may be beneficial to the restoration of elastic properties of cholesterol-manipulated cells.",
author = "Lai, {Wen Hsuan} and Jiunn-Der Liao and Shyh-Hau Wang",
year = "2012",
doi = "10.1109/ULTSYM.2012.0149",
language = "English",
isbn = "9781467345613",
pages = "600--603",
booktitle = "2012 IEEE International Ultrasonics Symposium, IUS 2012",

}

Lai, WH, Liao, J-D & Wang, S-H 2012, Effect of low-intensity pulsed ultrasound on the nano-mechanical properties of cholesterol-manipulated cells. in 2012 IEEE International Ultrasonics Symposium, IUS 2012., 6562337, pp. 600-603, 2012 IEEE International Ultrasonics Symposium, IUS 2012, Dresden, Germany, 12-10-07. https://doi.org/10.1109/ULTSYM.2012.0149

Effect of low-intensity pulsed ultrasound on the nano-mechanical properties of cholesterol-manipulated cells. / Lai, Wen Hsuan; Liao, Jiunn-Der; Wang, Shyh-Hau.

2012 IEEE International Ultrasonics Symposium, IUS 2012. 2012. p. 600-603 6562337.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

TY - GEN

T1 - Effect of low-intensity pulsed ultrasound on the nano-mechanical properties of cholesterol-manipulated cells

AU - Lai, Wen Hsuan

AU - Liao, Jiunn-Der

AU - Wang, Shyh-Hau

PY - 2012

Y1 - 2012

N2 - The mechanical properties of cells have been verified to correlate to human diseases and aging as well as to various cellular physiological processes, including proliferation, migration, and differentiation. It thus is essential to investigate responses of cells corresponding to chemical and physical stimulations for cellular mechanics studies and other applications. Moreover, measurement of the stiffness of an interested cell is of great importance to comprehend the cellular response with respect to a specific biophysical or biochemical alteration. Hence, current study aims to investigate the effect of low-intensity pulsed ultrasound (LIPUS) on nano-mechanical properties of cholesterol-manipulated cells. The NIH-3T3 fibroblastswere firstly incubated in cholesterol depletion solution. The cells were then insonified by a 1 MHz LIPUS for three minutes during the process of cholesterol restoration. In situ observation on the variations of living cells adhered on the glass substrate was monitored continuously. The cellular morphological changes were recorded for 8 hours. The nano-mechanical properties of cells were detected with nano-indentation by measuring the cellular membrane of a single cell. The cell properties seemed to be unchanged when adding with serum. On the other hand, the cholesterol depletion tended to lead a majority of cells to retract and become rounding corresponding to the increase of incubation time. The complications may be alleviated with the insonification of LIPUS. The elastic properties of those cholesterol-manipulated cells following LIPUS insonification tended to be improved. This study demonstrates that the variations of nano-mechanical property of cell membrane during cholesterol restoration and LIPUS insonification may be sensitively detected using the depth-sensing nano-indentation technique. The LIPUS insonification may be beneficial to the restoration of elastic properties of cholesterol-manipulated cells.

AB - The mechanical properties of cells have been verified to correlate to human diseases and aging as well as to various cellular physiological processes, including proliferation, migration, and differentiation. It thus is essential to investigate responses of cells corresponding to chemical and physical stimulations for cellular mechanics studies and other applications. Moreover, measurement of the stiffness of an interested cell is of great importance to comprehend the cellular response with respect to a specific biophysical or biochemical alteration. Hence, current study aims to investigate the effect of low-intensity pulsed ultrasound (LIPUS) on nano-mechanical properties of cholesterol-manipulated cells. The NIH-3T3 fibroblastswere firstly incubated in cholesterol depletion solution. The cells were then insonified by a 1 MHz LIPUS for three minutes during the process of cholesterol restoration. In situ observation on the variations of living cells adhered on the glass substrate was monitored continuously. The cellular morphological changes were recorded for 8 hours. The nano-mechanical properties of cells were detected with nano-indentation by measuring the cellular membrane of a single cell. The cell properties seemed to be unchanged when adding with serum. On the other hand, the cholesterol depletion tended to lead a majority of cells to retract and become rounding corresponding to the increase of incubation time. The complications may be alleviated with the insonification of LIPUS. The elastic properties of those cholesterol-manipulated cells following LIPUS insonification tended to be improved. This study demonstrates that the variations of nano-mechanical property of cell membrane during cholesterol restoration and LIPUS insonification may be sensitively detected using the depth-sensing nano-indentation technique. The LIPUS insonification may be beneficial to the restoration of elastic properties of cholesterol-manipulated cells.

UR - http://www.scopus.com/inward/record.url?scp=84882403637&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84882403637&partnerID=8YFLogxK

U2 - 10.1109/ULTSYM.2012.0149

DO - 10.1109/ULTSYM.2012.0149

M3 - Conference contribution

SN - 9781467345613

SP - 600

EP - 603

BT - 2012 IEEE International Ultrasonics Symposium, IUS 2012

ER -