Effect of low intensity ultrasounds on the growth of osteoblasts

Show Huie Chen, Chun Yi Chiu, Jui Ming Yeh, Shyh-Hau Wang

Research output: Chapter in Book/Report/Conference proceedingConference contribution

3 Citations (Scopus)

Abstract

Previous studies have indicated that low intensity ultrasounds could accelerate the repair of fibula fracture and facilitate the proliferation of osteoblasts. To further investigate the effect of low intensity ultrasounds, two different frequency of ultrasound, 1 MHz and 3 MHz, were applied to osteoblasts. The cells were stimulated for a typical 20% duty cycle of ultrasound with various intensities ranged from 50 to 150 mW/cm2 (ISATA) for 3 minutes once daily for 6 days. The cellular responses, in terms of cell number and morphological change, associated with ultrasound stimulations were estimated using a hemocytometer and microscopic morphology in which cells were stained with trypan blue. Results showed that the proliferation rates of osteoblasts for particular stimulated group are larger than those of control groups. The largest proliferation rate corresponds to those cells culture at 24 hours after seeding. Ultrasound increased approximately the cell proliferation proportional to ultrasonic intensity at which the exposure of 100 mW/cm 2 intensity of 1 MHz ultrasound 1.1 fold, and 50 mW/cm2 intensity of 3 MHz ultrasound 1.2 fold than that of the control group. These finding suggest that the growth of cell may be controlled by appropriate ultrasonic mechanical stress.

Original languageEnglish
Title of host publication29th Annual International Conference of IEEE-EMBS, Engineering in Medicine and Biology Society, EMBC'07
Pages5833-5836
Number of pages4
DOIs
Publication statusPublished - 2007 Dec 1
Event29th Annual International Conference of IEEE-EMBS, Engineering in Medicine and Biology Society, EMBC'07 - Lyon, France
Duration: 2007 Aug 232007 Aug 26

Publication series

NameAnnual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
ISSN (Print)0589-1019

Other

Other29th Annual International Conference of IEEE-EMBS, Engineering in Medicine and Biology Society, EMBC'07
CountryFrance
CityLyon
Period07-08-2307-08-26

All Science Journal Classification (ASJC) codes

  • Signal Processing
  • Biomedical Engineering
  • Computer Vision and Pattern Recognition
  • Health Informatics

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