Effect of microwave irradiation time on microwave-assisted weak acid protein hydrolysis

Dahee Kim, Minhee Joo, Dabin Lee, Huu Quang Nguyen, Jeongkwon Kim

Research output: Contribution to journalArticle

Abstract

Horse heart myoglobin (MYG) and bovine serum albumin (BSA) were hydrolyzed by microwave-assisted weak-acid hydrolysis for 10, 20, 30, 40, 50, and 60 min using 2% formic acid (FA) at 100°C. Generally, the number of identified peptides increased with increasing irradiation time, indicating that the duration of microwave irradiation is linked to the efficiency of hydrolysis. For MYG, irradiation for 60 min provided the highest number of identified peptides, the greatest sequence coverage values and the highest MASCOT score values among the investigated irradiation times. Irradiation of BSA for 50 min, however, yielded a greater number of peptides than irradiation for 60 min due to the generation of miscleaved peptides after microwave irradiation for 50 min.

Original languageEnglish
Pages (from-to)79-83
Number of pages5
JournalMass Spectrometry Letters
Volume10
Issue number3
DOIs
Publication statusPublished - 2019 Sep

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All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Spectroscopy

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