Effect of ultrasonic intensity on the Amyloid-β induced apoptosis of PC-12 cells

June Y. Chiu, Shyh-Hau Wang, Show H. Chen, Wen Tyng Li

Research output: Chapter in Book/Report/Conference proceedingConference contribution

3 Citations (Scopus)

Abstract

Previous studies have shown that the effect of Amyloid-β (Aβ) on the induction of apoptosis could lead neuronal loss to the Alzheimer's disease. The stimulation by physical energy, such as optics, was found able to greatly diminish Aβ induced apoptosis. This study is to further explore the effect of different ultrasonic energies on the neuronal cells. The experiments were carried out using such precursor neuronal cells as PC-12 which was added with Aβ of a 20 μM concentration during pre-cultured preparation. These cells were subsequently stimulated by a 20% duty cycle ultrasound of different energies ranged from 50 to 150 mW(SATA) for three minutes. The cellular response under ultrasound exposure was assessed, 7 times within 48 hours after those cells were stimulated, using the microscopic morphology, cell death measured by the typical MTT assay, and DNA fragmentation assay. Results showed that stimulations by ultrasound intensities of lower and higher than 100 mW/cm2 may respectively diminish and increase Aβ induced apoptosis compared to those control groups. Morphological results indicated that those cells stimulated by higher intensity ultrasounds tended to activate more differentiations in PC-12 discerned by morphological changes. This study demonstrated that the proliferation to diminish apoptosis and differentiation of neuronal cells could be regulated with the insonation of an appropriate ultrasonic intensity.

Original languageEnglish
Title of host publication2005 IEEE Ultrasonics Symposium
Pages1743-1746
Number of pages4
Volume3
DOIs
Publication statusPublished - 2005
Event2005 IEEE Ultrasonics Symposium - Rotterdam, Netherlands
Duration: 2005 Sep 182005 Sep 21

Other

Other2005 IEEE Ultrasonics Symposium
CountryNetherlands
CityRotterdam
Period05-09-1805-09-21

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Cell death
Ultrasonics
Assays
Amyloid
Optics
DNA
Cells
Experiments

All Science Journal Classification (ASJC) codes

  • Engineering(all)

Cite this

Chiu, J. Y., Wang, S-H., Chen, S. H., & Li, W. T. (2005). Effect of ultrasonic intensity on the Amyloid-β induced apoptosis of PC-12 cells. In 2005 IEEE Ultrasonics Symposium (Vol. 3, pp. 1743-1746). [1603203] https://doi.org/10.1109/ULTSYM.2005.1603203
Chiu, June Y. ; Wang, Shyh-Hau ; Chen, Show H. ; Li, Wen Tyng. / Effect of ultrasonic intensity on the Amyloid-β induced apoptosis of PC-12 cells. 2005 IEEE Ultrasonics Symposium. Vol. 3 2005. pp. 1743-1746
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Chiu, JY, Wang, S-H, Chen, SH & Li, WT 2005, Effect of ultrasonic intensity on the Amyloid-β induced apoptosis of PC-12 cells. in 2005 IEEE Ultrasonics Symposium. vol. 3, 1603203, pp. 1743-1746, 2005 IEEE Ultrasonics Symposium, Rotterdam, Netherlands, 05-09-18. https://doi.org/10.1109/ULTSYM.2005.1603203

Effect of ultrasonic intensity on the Amyloid-β induced apoptosis of PC-12 cells. / Chiu, June Y.; Wang, Shyh-Hau; Chen, Show H.; Li, Wen Tyng.

2005 IEEE Ultrasonics Symposium. Vol. 3 2005. p. 1743-1746 1603203.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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Chiu JY, Wang S-H, Chen SH, Li WT. Effect of ultrasonic intensity on the Amyloid-β induced apoptosis of PC-12 cells. In 2005 IEEE Ultrasonics Symposium. Vol. 3. 2005. p. 1743-1746. 1603203 https://doi.org/10.1109/ULTSYM.2005.1603203