Effect of various ultrasound insonifications on the Aβ-induced apoptotic neurons

Chun Yi Chiu, Shyh-Hau Wang, Show Huie Chen

Research output: Chapter in Book/Report/Conference proceedingConference contribution


Amyloid beta (Aβ) is a peptide that was known able to induce the apoptosis of nerve cells in the brain leading to the development of Alzheimer's disease (AD). In addition to providing medication treatment for the neurodegenerative disease, previous cell culture studies applying physical stimulations of a certain optical energy or low intensity ultrasound on the apoptotic cells have demonstrated that the viability of Ab-induced apoptotic neurons could be increased. Current study is to further explore insonification effects, such as frequency, exposure duration, and intensity on the apoptotic nerve cells and that the result is to provide a basic understanding about the therapeutic effects of ultrasound on the Ab-induced apoptotic neurons. Experiment were carried out using neuronal PC12 cells that were insonified by 1 MHz ultrasound with 20% duty cycle, 150 mW/cm2 (SATA) for 3 minutes. After 72 hours, the viability and fate of cells were respectively evaluated by MTT assay and annexin V/PI double stain assay. Results showed that the stimulations by low intensity ultrasounds may contribute to the reduction of A-b induced cell apoptosis or necrosis. The effect of decreasing apoptotic cells tends to be apparent at a certain time depending on the condition of insonified ultrasounds. The variation of temperature in the cell plate was within 1°C throughout each insonification procedure. Thus, the effect on apoptotic PC12 cells may be largely due to the non- thermal effects of insonified ultrasound, which could enhance the permeability and other signal transduction pathways for cells.

Original languageEnglish
Title of host publication2007 IEEE Ultrasonics Symposium Proceedings, IUS
Number of pages4
Publication statusPublished - 2007 Dec 1
Event2007 IEEE Ultrasonics Symposium, IUS - New York, NY, United States
Duration: 2007 Oct 282007 Oct 31

Publication series

NameProceedings - IEEE Ultrasonics Symposium
ISSN (Print)1051-0117


Other2007 IEEE Ultrasonics Symposium, IUS
Country/TerritoryUnited States
CityNew York, NY

All Science Journal Classification (ASJC) codes

  • Acoustics and Ultrasonics


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