Effective perturbations on the amplitude and hysteresis of erg-mediated potassium current caused by 1-octylnonyl 8-[(2-hydroxyethyl)[6-oxo-6(undecyloxy)hexyl]amino]-octanoate (sm-102), a cationic lipid

SM-102 (1-octylnonyl 8-[(2-hydroxyethyl)[6-oxo-6-(undecyloxy)hexyl]amino]-octanoate) is an amino cationic lipid that has been tailored for the formation of lipid nanoparticles and it is one of the essential ingredients present in the Moderna™ COVID-19 vaccine. However, to what extent it may modify varying types of plasmalemmal ionic currents remains largely uncertain. In this study, we investigate the effects of SM-102 on ionic currents either in two types of endocrine cells (e.g., rat pituitary tumor (GH₃) cells and mouse Leydig tumor (MA-10) cells) or in microglial (BV2) cells. Hyperpolarization-activated K⁺ currents in these cells bathed in high-K⁺, Ca²⁺-free extracellular solution were examined to assess the effects of SM-102 on the amplitude and hysteresis of the erg-mediated K⁺ current (I_K(erg)). The SM-102 addition was effective at blocking I_K(erg) in a concentration-dependent fashion with a half-maximal concentration (IC₅₀) of 108 µM, a value which is similar to the K_D value (i.e., 134 µM) required for its accentuation of deactivation time constant of the current. The hysteretic strength of I_K(erg) in response to the long-lasting isosceles-triangular ramp pulse was effectively decreased in the presence of SM-102. Cell exposure to TurboFectin™ 8.0 (0.1%, v/v), a transfection reagent, was able to inhibit hyperpolarization-activated I_K(erg) effectively with an increase in the deactivation time course of the current. Additionally, in GH₃ cells dialyzed with spermine (30 µM), the I_K(erg) amplitude progressively decreased; moreover, a further bath application of SM-102 (100 µM) or TurboFectin (0.1%) diminished the current magnitude further. In MA-10 Leydig cells, the I_K(erg) was also blocked by the presence of SM-102 or TurboFectin. The IC₅₀ value for SM-102-induced inhibition of I_K(erg) in MA-10 cells was 98 µM. In BV2 microglial cells, the amplitude of the inwardly rectifying K⁺ current was inhibited by SM-102. Taken together, the presence of SM-102 concentration-dependently inhibited I_K(erg) in endocrine cells (e.g., GH₃ or MA-10 cells), and such action may contribute to their functional activities, assuming that similar in vivo findings exist.