Abstract
Amphetamine influences plasma and testicular testosterone levels. However, there is no evidence that amphetamine can directly influence Leydig cell functions. In the present study, a MA-10 mouse Leydig tumor cell line was used to determine whether and how amphetamine affected Leydig cell steroidogenesis. MA-10 cells were treated with different concentrations of amphetamine without or with human chorionic gonadotropin (hCG) and/or enzyme precursors over different time durations. Steroid production, enzyme activities and StAR protein expression were determined. Amphetamine alone had no any effect on MA-10 cell steroidogenesis. However, amphetamine (10-11M and 10-10M) significantly enhanced hCG-treated progesterone production at 3 hr in MA-10 cells (p < 0.05). Furthermore, amphetamine significantly induced more progesterone production upon treatment with 22R-hydroxycholesterol (p < 0.05), a precursor of P450 side-chain cleavage enzyme (P450scc). However, amphetamine did not induce more progesterone production when treated with pregnenolone (p > 0.05), a precursor of 3β-hydroxysteroid dehydrogenase. In addition, the expressions of StAR protein and P450scc enzyme were not significantly different between hCG alone and hCG plus amphetamine treatment in MA-10 cells (p > 0.05). These results suggested that amphetamine enhanced hCG-induced progesterone production in MA-10 cells by increasing P450scc activity without influencng StAR protein and P450scc enzyme expression or 3β-HSD enzyme activity.
| Original language | English |
|---|---|
| Pages (from-to) | 1983-1995 |
| Number of pages | 13 |
| Journal | Life Sciences |
| Volume | 72 |
| Issue number | 17 |
| DOIs | |
| Publication status | Published - 2003 Mar 14 |
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All Science Journal Classification (ASJC) codes
- Biochemistry, Genetics and Molecular Biology(all)
- Pharmacology, Toxicology and Pharmaceutics(all)
Cite this
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Effects of amphetamine on steroidogenesis in MA-10 mouse Leydig tumor cells. / Chen, Liang Yu; Huang, Yuan Li; Liu, Ming-Yi; Leu, Sew Fen; Huang, Bu-Miin.
In: Life Sciences, Vol. 72, No. 17, 14.03.2003, p. 1983-1995.Research output: Contribution to journal › Article
TY - JOUR
T1 - Effects of amphetamine on steroidogenesis in MA-10 mouse Leydig tumor cells
AU - Chen, Liang Yu
AU - Huang, Yuan Li
AU - Liu, Ming-Yi
AU - Leu, Sew Fen
AU - Huang, Bu-Miin
PY - 2003/3/14
Y1 - 2003/3/14
N2 - Amphetamine influences plasma and testicular testosterone levels. However, there is no evidence that amphetamine can directly influence Leydig cell functions. In the present study, a MA-10 mouse Leydig tumor cell line was used to determine whether and how amphetamine affected Leydig cell steroidogenesis. MA-10 cells were treated with different concentrations of amphetamine without or with human chorionic gonadotropin (hCG) and/or enzyme precursors over different time durations. Steroid production, enzyme activities and StAR protein expression were determined. Amphetamine alone had no any effect on MA-10 cell steroidogenesis. However, amphetamine (10-11M and 10-10M) significantly enhanced hCG-treated progesterone production at 3 hr in MA-10 cells (p < 0.05). Furthermore, amphetamine significantly induced more progesterone production upon treatment with 22R-hydroxycholesterol (p < 0.05), a precursor of P450 side-chain cleavage enzyme (P450scc). However, amphetamine did not induce more progesterone production when treated with pregnenolone (p > 0.05), a precursor of 3β-hydroxysteroid dehydrogenase. In addition, the expressions of StAR protein and P450scc enzyme were not significantly different between hCG alone and hCG plus amphetamine treatment in MA-10 cells (p > 0.05). These results suggested that amphetamine enhanced hCG-induced progesterone production in MA-10 cells by increasing P450scc activity without influencng StAR protein and P450scc enzyme expression or 3β-HSD enzyme activity.
AB - Amphetamine influences plasma and testicular testosterone levels. However, there is no evidence that amphetamine can directly influence Leydig cell functions. In the present study, a MA-10 mouse Leydig tumor cell line was used to determine whether and how amphetamine affected Leydig cell steroidogenesis. MA-10 cells were treated with different concentrations of amphetamine without or with human chorionic gonadotropin (hCG) and/or enzyme precursors over different time durations. Steroid production, enzyme activities and StAR protein expression were determined. Amphetamine alone had no any effect on MA-10 cell steroidogenesis. However, amphetamine (10-11M and 10-10M) significantly enhanced hCG-treated progesterone production at 3 hr in MA-10 cells (p < 0.05). Furthermore, amphetamine significantly induced more progesterone production upon treatment with 22R-hydroxycholesterol (p < 0.05), a precursor of P450 side-chain cleavage enzyme (P450scc). However, amphetamine did not induce more progesterone production when treated with pregnenolone (p > 0.05), a precursor of 3β-hydroxysteroid dehydrogenase. In addition, the expressions of StAR protein and P450scc enzyme were not significantly different between hCG alone and hCG plus amphetamine treatment in MA-10 cells (p > 0.05). These results suggested that amphetamine enhanced hCG-induced progesterone production in MA-10 cells by increasing P450scc activity without influencng StAR protein and P450scc enzyme expression or 3β-HSD enzyme activity.
UR - http://www.scopus.com/inward/record.url?scp=0037436751&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0037436751&partnerID=8YFLogxK
U2 - 10.1016/S0024-3205(03)00011-0
DO - 10.1016/S0024-3205(03)00011-0
M3 - Article
C2 - 12597997
AN - SCOPUS:0037436751
VL - 72
SP - 1983
EP - 1995
JO - Life Sciences
JF - Life Sciences
SN - 0024-3205
IS - 17
ER -