TY - JOUR
T1 - Effects of Ba-Wei-Die-Huang-Wan on the cholinergic function and protein expression of M2 muscarinic receptor of the urinary bladder in diabetic rats
AU - Tong, Yat Ching
AU - Cheng, Juei Tang
AU - Wan, Wang Chuan
N1 - Funding Information:
We acknowledge Cheng-Hoo Pharmaceutical Co. (Tainan, Taiwan) for the kind supply of granules of Bawei-DHW. The present study was supported in part by grants from the National Science Council, Taiwan, ROC (NSC-89-2314-B006-143 and NSC-90-2314-B006-161).
PY - 2002/9/13
Y1 - 2002/9/13
N2 - The study investigated the effect of a traditional Chinese herbal preparation 'Ba-Wei-Die-Huang-Wan' on the cholinergic function of the urinary bladder in diabetic rats, as well as its influence on the protein expression of muscarinic M2 receptors. Wistar rats were divided into three groups: (1) control rats; (2) 2-week streptozotocin-induced diabetic rats; and (3) diabetic rats treated with Ba-Wei-Die-Huang-Wan 26 mg/kg thrice daily for 3 days. A dose-response study was performed for bladder muscle strip contractile response to a muscarinic agonist, arecaidine propargyl ester (APE). The amount of M2 receptor protein in the bladder was measured by Western immunoblotting using monoclonal antibodies. The bladder contractile response to APE was significantly increased in the diabetic rats compared to the control. The M2 receptor protein density was also significantly higher by 106% (P<0.01, n=8) in diabetes. Ba-Wei-Die-Huang-Wan treatment significantly lowered the plasma glucose level of the diabetic rats. The increases in contractile response to APE as well as the M2 receptor protein density were partially reversed by the treatment. In conclusion, there was an over-expression of M2 receptor resulting in hyper-contractility in the bladder of diabetic rats. Ba-Wei-Die-Huang-Wan significantly alleviated the alterations.
AB - The study investigated the effect of a traditional Chinese herbal preparation 'Ba-Wei-Die-Huang-Wan' on the cholinergic function of the urinary bladder in diabetic rats, as well as its influence on the protein expression of muscarinic M2 receptors. Wistar rats were divided into three groups: (1) control rats; (2) 2-week streptozotocin-induced diabetic rats; and (3) diabetic rats treated with Ba-Wei-Die-Huang-Wan 26 mg/kg thrice daily for 3 days. A dose-response study was performed for bladder muscle strip contractile response to a muscarinic agonist, arecaidine propargyl ester (APE). The amount of M2 receptor protein in the bladder was measured by Western immunoblotting using monoclonal antibodies. The bladder contractile response to APE was significantly increased in the diabetic rats compared to the control. The M2 receptor protein density was also significantly higher by 106% (P<0.01, n=8) in diabetes. Ba-Wei-Die-Huang-Wan treatment significantly lowered the plasma glucose level of the diabetic rats. The increases in contractile response to APE as well as the M2 receptor protein density were partially reversed by the treatment. In conclusion, there was an over-expression of M2 receptor resulting in hyper-contractility in the bladder of diabetic rats. Ba-Wei-Die-Huang-Wan significantly alleviated the alterations.
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U2 - 10.1016/S0304-3940(02)00713-9
DO - 10.1016/S0304-3940(02)00713-9
M3 - Article
C2 - 12213625
AN - SCOPUS:0037072570
VL - 330
SP - 21
EP - 24
JO - Neuroscience Letters
JF - Neuroscience Letters
SN - 0304-3940
IS - 1
ER -
