Effects of Ba-Wei-Die-Huang-Wan on the cholinergic function and protein expression of M2 muscarinic receptor of the urinary bladder in diabetic rats

Yat-Ching Tong, Juei Tang Cheng, Wang Chuan Wan

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

The study investigated the effect of a traditional Chinese herbal preparation 'Ba-Wei-Die-Huang-Wan' on the cholinergic function of the urinary bladder in diabetic rats, as well as its influence on the protein expression of muscarinic M2 receptors. Wistar rats were divided into three groups: (1) control rats; (2) 2-week streptozotocin-induced diabetic rats; and (3) diabetic rats treated with Ba-Wei-Die-Huang-Wan 26 mg/kg thrice daily for 3 days. A dose-response study was performed for bladder muscle strip contractile response to a muscarinic agonist, arecaidine propargyl ester (APE). The amount of M2 receptor protein in the bladder was measured by Western immunoblotting using monoclonal antibodies. The bladder contractile response to APE was significantly increased in the diabetic rats compared to the control. The M2 receptor protein density was also significantly higher by 106% (P<0.01, n=8) in diabetes. Ba-Wei-Die-Huang-Wan treatment significantly lowered the plasma glucose level of the diabetic rats. The increases in contractile response to APE as well as the M2 receptor protein density were partially reversed by the treatment. In conclusion, there was an over-expression of M2 receptor resulting in hyper-contractility in the bladder of diabetic rats. Ba-Wei-Die-Huang-Wan significantly alleviated the alterations.

Original languageEnglish
Pages (from-to)21-24
Number of pages4
JournalNeuroscience Letters
Volume330
Issue number1
DOIs
Publication statusPublished - 2002 Sep 13

Fingerprint

Muscarinic M2 Receptors
Cholinergic Agents
Urinary Bladder
Proteins
Plant Preparations
Muscarinic Agonists
Streptozocin
die-huang-wan
Wistar Rats
Western Blotting
Monoclonal Antibodies
Glucose
Muscles
Control Groups

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

Cite this

@article{8d1fa1843b98484dad6d895ac07deef3,
title = "Effects of Ba-Wei-Die-Huang-Wan on the cholinergic function and protein expression of M2 muscarinic receptor of the urinary bladder in diabetic rats",
abstract = "The study investigated the effect of a traditional Chinese herbal preparation 'Ba-Wei-Die-Huang-Wan' on the cholinergic function of the urinary bladder in diabetic rats, as well as its influence on the protein expression of muscarinic M2 receptors. Wistar rats were divided into three groups: (1) control rats; (2) 2-week streptozotocin-induced diabetic rats; and (3) diabetic rats treated with Ba-Wei-Die-Huang-Wan 26 mg/kg thrice daily for 3 days. A dose-response study was performed for bladder muscle strip contractile response to a muscarinic agonist, arecaidine propargyl ester (APE). The amount of M2 receptor protein in the bladder was measured by Western immunoblotting using monoclonal antibodies. The bladder contractile response to APE was significantly increased in the diabetic rats compared to the control. The M2 receptor protein density was also significantly higher by 106{\%} (P<0.01, n=8) in diabetes. Ba-Wei-Die-Huang-Wan treatment significantly lowered the plasma glucose level of the diabetic rats. The increases in contractile response to APE as well as the M2 receptor protein density were partially reversed by the treatment. In conclusion, there was an over-expression of M2 receptor resulting in hyper-contractility in the bladder of diabetic rats. Ba-Wei-Die-Huang-Wan significantly alleviated the alterations.",
author = "Yat-Ching Tong and Cheng, {Juei Tang} and Wan, {Wang Chuan}",
year = "2002",
month = "9",
day = "13",
doi = "10.1016/S0304-3940(02)00713-9",
language = "English",
volume = "330",
pages = "21--24",
journal = "Neuroscience Letters",
issn = "0304-3940",
publisher = "Elsevier Ireland Ltd",
number = "1",

}

Effects of Ba-Wei-Die-Huang-Wan on the cholinergic function and protein expression of M2 muscarinic receptor of the urinary bladder in diabetic rats. / Tong, Yat-Ching; Cheng, Juei Tang; Wan, Wang Chuan.

In: Neuroscience Letters, Vol. 330, No. 1, 13.09.2002, p. 21-24.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Effects of Ba-Wei-Die-Huang-Wan on the cholinergic function and protein expression of M2 muscarinic receptor of the urinary bladder in diabetic rats

AU - Tong, Yat-Ching

AU - Cheng, Juei Tang

AU - Wan, Wang Chuan

PY - 2002/9/13

Y1 - 2002/9/13

N2 - The study investigated the effect of a traditional Chinese herbal preparation 'Ba-Wei-Die-Huang-Wan' on the cholinergic function of the urinary bladder in diabetic rats, as well as its influence on the protein expression of muscarinic M2 receptors. Wistar rats were divided into three groups: (1) control rats; (2) 2-week streptozotocin-induced diabetic rats; and (3) diabetic rats treated with Ba-Wei-Die-Huang-Wan 26 mg/kg thrice daily for 3 days. A dose-response study was performed for bladder muscle strip contractile response to a muscarinic agonist, arecaidine propargyl ester (APE). The amount of M2 receptor protein in the bladder was measured by Western immunoblotting using monoclonal antibodies. The bladder contractile response to APE was significantly increased in the diabetic rats compared to the control. The M2 receptor protein density was also significantly higher by 106% (P<0.01, n=8) in diabetes. Ba-Wei-Die-Huang-Wan treatment significantly lowered the plasma glucose level of the diabetic rats. The increases in contractile response to APE as well as the M2 receptor protein density were partially reversed by the treatment. In conclusion, there was an over-expression of M2 receptor resulting in hyper-contractility in the bladder of diabetic rats. Ba-Wei-Die-Huang-Wan significantly alleviated the alterations.

AB - The study investigated the effect of a traditional Chinese herbal preparation 'Ba-Wei-Die-Huang-Wan' on the cholinergic function of the urinary bladder in diabetic rats, as well as its influence on the protein expression of muscarinic M2 receptors. Wistar rats were divided into three groups: (1) control rats; (2) 2-week streptozotocin-induced diabetic rats; and (3) diabetic rats treated with Ba-Wei-Die-Huang-Wan 26 mg/kg thrice daily for 3 days. A dose-response study was performed for bladder muscle strip contractile response to a muscarinic agonist, arecaidine propargyl ester (APE). The amount of M2 receptor protein in the bladder was measured by Western immunoblotting using monoclonal antibodies. The bladder contractile response to APE was significantly increased in the diabetic rats compared to the control. The M2 receptor protein density was also significantly higher by 106% (P<0.01, n=8) in diabetes. Ba-Wei-Die-Huang-Wan treatment significantly lowered the plasma glucose level of the diabetic rats. The increases in contractile response to APE as well as the M2 receptor protein density were partially reversed by the treatment. In conclusion, there was an over-expression of M2 receptor resulting in hyper-contractility in the bladder of diabetic rats. Ba-Wei-Die-Huang-Wan significantly alleviated the alterations.

UR - http://www.scopus.com/inward/record.url?scp=0037072570&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037072570&partnerID=8YFLogxK

U2 - 10.1016/S0304-3940(02)00713-9

DO - 10.1016/S0304-3940(02)00713-9

M3 - Article

C2 - 12213625

AN - SCOPUS:0037072570

VL - 330

SP - 21

EP - 24

JO - Neuroscience Letters

JF - Neuroscience Letters

SN - 0304-3940

IS - 1

ER -