TY - JOUR
T1 - Effects of cultivation conditions and media composition on cell growth and lipid productivity of indigenous microalga Chlorella vulgaris ESP-31
AU - Yeh, Kuei Ling
AU - Chang, Jo Shu
N1 - Funding Information:
The authors gratefully acknowledge financial support from Taiwan’s National Science Council under grant numbers NSC 100-3113-E-006-016- and NSC 99-3113-P-110-001- . The support from the Top University grant of National Cheng Kung University is also appreciated.
PY - 2012/2
Y1 - 2012/2
N2 - The growth and lipid productivity of an isolated microalga Chlorella vulgaris ESP-31 were investigated under different media and cultivation conditions, including phototrophic growth (NaHCO 3 or CO 2, with light), heterotrophic growth (glucose, without light), photoheterotrophic growth (glucose, with light) and mixotrophic growth (glucose and CO 2, with light). C. vulgaris ESP-31 preferred to grow under phototrophic (CO 2), photoheterotrophic and mixotrophic conditions on nitrogen-rich medium (i.e., Basal medium and Modified Bristol's medium), reaching a biomass concentration of 2-5g/l. The growth on nitrogen-limiting MBL medium resulted in higher lipid accumulation (20-53%) but slower growth rate. Higher lipid content (40-53%) and higher lipid productivity (67-144mg/l/d) were obtained under mixotrophic cultivation with all the culture media used. The fatty acid composition of the microalgal lipid comprises over 60-68% of saturated fatty acids (i.e., palmitic acid (C16:0), stearic acid (C18:0)) and monounsaturated acids (i.e., oleic acid (C18:1)). This lipid composition is suitable for biodiesel production.
AB - The growth and lipid productivity of an isolated microalga Chlorella vulgaris ESP-31 were investigated under different media and cultivation conditions, including phototrophic growth (NaHCO 3 or CO 2, with light), heterotrophic growth (glucose, without light), photoheterotrophic growth (glucose, with light) and mixotrophic growth (glucose and CO 2, with light). C. vulgaris ESP-31 preferred to grow under phototrophic (CO 2), photoheterotrophic and mixotrophic conditions on nitrogen-rich medium (i.e., Basal medium and Modified Bristol's medium), reaching a biomass concentration of 2-5g/l. The growth on nitrogen-limiting MBL medium resulted in higher lipid accumulation (20-53%) but slower growth rate. Higher lipid content (40-53%) and higher lipid productivity (67-144mg/l/d) were obtained under mixotrophic cultivation with all the culture media used. The fatty acid composition of the microalgal lipid comprises over 60-68% of saturated fatty acids (i.e., palmitic acid (C16:0), stearic acid (C18:0)) and monounsaturated acids (i.e., oleic acid (C18:1)). This lipid composition is suitable for biodiesel production.
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U2 - 10.1016/j.biortech.2011.11.103
DO - 10.1016/j.biortech.2011.11.103
M3 - Article
C2 - 22189073
AN - SCOPUS:84855217195
SN - 0960-8524
VL - 105
SP - 120
EP - 127
JO - Bioresource technology
JF - Bioresource technology
ER -