TY - JOUR
T1 - Effects of inorganic nutrient levels on the biodegradation of benzene, toluene, and xylene (BTX) by Pseudomonas spp. in a laboratory porous media sand aquifer model
AU - Jean, Jiin Shuh
AU - Lee, Ming Kuo
AU - Wang, Shih Ming
AU - Chattopadhyay, Pabitra
AU - Maity, Jyoti Prakash
N1 - Funding Information:
This work was supported by grant NSC86-2116-M006-009 from the National Science Council in Taiwan and Top100 University Advancement Office, National Cheng Kung University, Tainan, Taiwan. We would like to thank Professor I-Cheng Tseng from the Department of Life Sciences of National Cheng Kung University for his valuable comments to this study. We also thank Mr. Chia-Chuan Liu and Mr. Jiann-Hong Liu at the Department of Earth Sciences of National Cheng Kung University to measure the experimental errors of ion chromatography and gas chromatography.
PY - 2008/11
Y1 - 2008/11
N2 - The effect of inorganic nutrients (sulfate, phosphate, and ammonium chloride) on the aerobic biodegradation of benzene, toluene, and xylene (BTX) by Pseudomonas spp. was studied in the laboratory using a glass sand tank. The increase of nutrient levels resulted in enhanced bacterial growth and BTX degradation. Sulfate and phosphate serve as key electron acceptors in the microbiological processes degrading BTX. The observed bacterial morphological changes during BTX degradation reveal that the filamentous bacteria were the dominant species at low temperatures about 20 °C. The spherical and rod-shaped cells became dominant at higher temperatures ranging from 25 °C to 28 °C. When the BTX mixture was allowed to be biodegraded for longer incubation periods of 21-42 h at high phosphate concentrations, large amounts of rod-shaped cells were clustered. The morphological adaptation appears to be controlled by the temperature and nutrient levels in the sandy medium where Pseudomonas spp. thrives.
AB - The effect of inorganic nutrients (sulfate, phosphate, and ammonium chloride) on the aerobic biodegradation of benzene, toluene, and xylene (BTX) by Pseudomonas spp. was studied in the laboratory using a glass sand tank. The increase of nutrient levels resulted in enhanced bacterial growth and BTX degradation. Sulfate and phosphate serve as key electron acceptors in the microbiological processes degrading BTX. The observed bacterial morphological changes during BTX degradation reveal that the filamentous bacteria were the dominant species at low temperatures about 20 °C. The spherical and rod-shaped cells became dominant at higher temperatures ranging from 25 °C to 28 °C. When the BTX mixture was allowed to be biodegraded for longer incubation periods of 21-42 h at high phosphate concentrations, large amounts of rod-shaped cells were clustered. The morphological adaptation appears to be controlled by the temperature and nutrient levels in the sandy medium where Pseudomonas spp. thrives.
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U2 - 10.1016/j.biortech.2008.01.064
DO - 10.1016/j.biortech.2008.01.064
M3 - Article
C2 - 18329875
AN - SCOPUS:45449086666
VL - 99
SP - 7807
EP - 7815
JO - Agricultural Wastes
JF - Agricultural Wastes
SN - 0960-8524
IS - 16
ER -
