Effects of iron-cored carbonballs as matrix on matrix-assisted laser desorption ionization-time of flight mass spectrometer analysis

Hsiangshen Wen, Laifu Tsai, Chienwei Liu, Yuchang Tyan, Shyilong Shy, Bautong Dai, Paochi Liao, Chongyao Wang

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Mass spectrometry is an important technique for characterizing the structures of surfaces and has several characteristics that are especially valuable in the bioanalytical application. Matrix-assisted laser desorption ionization-time of flight mass spectrometer (MALDI-TOF-MS) is commonly used in preliminary protein identification. To prepare the sample for analysis, purified-proteins of cryptic digestion are carefully mixed with saturated liquid-phase matrices, classified as high-molecular-weight organic polymer, which are essential to promote analytical accuracy via mechanism of laser-energy transfer to peptides. Signals generated from matrix in the analytical process interfere with signals of small peptides in the same region of mass-to-charge ratio. In order to improve analytical results, we demonstrated that matrix in MALDI-TOF-MS can be replaced by carbon nanomaterial. We substituted iron-cored carbonballs for matrix well mixing with trypsinized bovine serum albumins (BSA) and performed mass spectrometrical analyses. Experimental results indicated that in addition to iron-cored carbonballs possessed equivalent functionality as matrix does, several improved aspects were also observed.

Original languageEnglish
Pages (from-to)6415-6418
Number of pages4
JournalJapanese Journal of Applied Physics
Volume46
Issue number9 B
DOIs
Publication statusPublished - 2007 Sept 20

All Science Journal Classification (ASJC) codes

  • General Engineering
  • General Physics and Astronomy

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