Effects of iron excess on cell viability and mitogen-activated protein kinase activation in rice roots

Tsung Mu Tsai, Hao-Jen Huang

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Iron is an essential micronutrient for normal growth and development of plants. However, at high concentrations, iron can become toxic to plants. Very little information is known about the molecular mechanism responsible for the regulation of plant growth by excess iron. The aim of this study was to investigate the signal transduction pathway activated by increasing concentrations (0.5, 1.0, and 2.5 mm) of iron. We showed that iron elicited a remarkable myelin basic protein (MBP) kinase activity. By Western blot and immunoprecipitation analysis, we suggested that iron-activated 42-kDa MBP kinase is a mitogen-activated protein kinase (MAPK). Cell death in rice roots due to iron toxicity was investigated using inhibitors of signal molecules known to regulate programmed cell death in plants. Phenylarsine oxide (PAO) and sodium orthovanadate, known inhibitors of tyrosine phosphatase, reduced iron-induced root cell death, but cantharidin and endothall two-serine/threonine phosphatase inhibitor enhanced iron-induced root cell death. Moreover, our results revealed that H+-ATPase might participate in iron-induced cell death. These results suggested that the MAPK, reactive oxygen species, protein phosphatase, and H+-ATPase might function in the plant iron-triggered signaling pathway in rice roots.

Original languageEnglish
Pages (from-to)583-592
Number of pages10
JournalPhysiologia plantarum
Volume127
Issue number4
DOIs
Publication statusPublished - 2006 Aug 1

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Mitogen-Activated Protein Kinases
mitogen-activated protein kinase
cell viability
Cell Survival
Iron
iron
rice
Cell Death
cell death
H-transporting ATP synthase
Proton-Translocating ATPases
Myelin Basic Protein
Phosphoprotein Phosphatases
protein kinases
Protein Kinases
Oryza
Cantharidin
cantharidin
Toxic Plants
Vanadates

All Science Journal Classification (ASJC) codes

  • Plant Science

Cite this

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abstract = "Iron is an essential micronutrient for normal growth and development of plants. However, at high concentrations, iron can become toxic to plants. Very little information is known about the molecular mechanism responsible for the regulation of plant growth by excess iron. The aim of this study was to investigate the signal transduction pathway activated by increasing concentrations (0.5, 1.0, and 2.5 mm) of iron. We showed that iron elicited a remarkable myelin basic protein (MBP) kinase activity. By Western blot and immunoprecipitation analysis, we suggested that iron-activated 42-kDa MBP kinase is a mitogen-activated protein kinase (MAPK). Cell death in rice roots due to iron toxicity was investigated using inhibitors of signal molecules known to regulate programmed cell death in plants. Phenylarsine oxide (PAO) and sodium orthovanadate, known inhibitors of tyrosine phosphatase, reduced iron-induced root cell death, but cantharidin and endothall two-serine/threonine phosphatase inhibitor enhanced iron-induced root cell death. Moreover, our results revealed that H+-ATPase might participate in iron-induced cell death. These results suggested that the MAPK, reactive oxygen species, protein phosphatase, and H+-ATPase might function in the plant iron-triggered signaling pathway in rice roots.",
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Effects of iron excess on cell viability and mitogen-activated protein kinase activation in rice roots. / Tsai, Tsung Mu; Huang, Hao-Jen.

In: Physiologia plantarum, Vol. 127, No. 4, 01.08.2006, p. 583-592.

Research output: Contribution to journalArticle

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