Effects of methionine restriction and endurance exercise on bones of ovariectomized rats

A study of histomorphometry, densitometry, and biomechanical properties

Tsang-Hai Huang, I. Hsiu Su, Jack L. Lewis, Ming-Shi Chang, Ar Tyan Hsu, Carmen E. Perrone, Gene P. Ables

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

To investigate the effects of dietary methionine restriction (MetR) and endurance exercise on bone quality under a condition of estrogen deficiency, female Sprague-Dawley rats (36-wk-old) were assigned to a sham surgery group or one of five ovariectomized groups subjected to interventions of no treatment (Ovx), endurance exercise (Exe), methionine restriction (MetR), methionine restriction plus endurance exercise (MetR + Exe), and estrogen treatment (Est). Rats in the exercise groups were subjected to a treadmill running regimen. MetR and control diets contained 0.172 and 0.86% methionine, respectively. After the 12-wk intervention, all animals were killed, and serum and bone tissues were collected for analyses. Compared with estrogen treatment, MetR diet and endurance exercise showed better or equivalent efficiency in reducing body weight gain caused by ovariectomy (P < 0.05). Whereas only the Est group showed evidence for reduced bone turnover compared with the Ovx group, MetR diet and/or endurance exercise demonstrated efficiencies in downregulating serum insulin, leptin, triglyceride, and thiobarbituric acid reactive substances (P < 0.05). Both the Exe and MetR groups showed higher femoral cortical and total volumetric bone mineral density (vBMD), but only the Exe and Est groups preserved cancellous bone volume and/or vBMD of distal femora (P < 0.05) compared with the Ovx group. After being normalized to body mass, femora of the MetR and MetR + Exe groups had relatively higher bending strength and dimension values followed by the Sham, Exe, and Est groups (P < 0.05). In conclusion, both MetR diet and endurance exercise improved cortical bone properties, but only endurance exercise preserved cancellous bone under estrogen deficiency.

Original languageEnglish
Pages (from-to)517-526
Number of pages10
JournalJournal of Applied Physiology
Volume119
Issue number5
DOIs
Publication statusPublished - 2015 Sep 1

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Densitometry
Methionine
Bone and Bones
Estrogens
Diet
Bone Density
Femur
Thiobarbituric Acid Reactive Substances
Bone Remodeling
Ovariectomy
Substance P
Leptin
Thigh
Serum
Running
Weight Gain
Sprague Dawley Rats
Triglycerides
Down-Regulation
Body Weight

All Science Journal Classification (ASJC) codes

  • Physiology
  • Physiology (medical)

Cite this

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title = "Effects of methionine restriction and endurance exercise on bones of ovariectomized rats: A study of histomorphometry, densitometry, and biomechanical properties",
abstract = "To investigate the effects of dietary methionine restriction (MetR) and endurance exercise on bone quality under a condition of estrogen deficiency, female Sprague-Dawley rats (36-wk-old) were assigned to a sham surgery group or one of five ovariectomized groups subjected to interventions of no treatment (Ovx), endurance exercise (Exe), methionine restriction (MetR), methionine restriction plus endurance exercise (MetR + Exe), and estrogen treatment (Est). Rats in the exercise groups were subjected to a treadmill running regimen. MetR and control diets contained 0.172 and 0.86{\%} methionine, respectively. After the 12-wk intervention, all animals were killed, and serum and bone tissues were collected for analyses. Compared with estrogen treatment, MetR diet and endurance exercise showed better or equivalent efficiency in reducing body weight gain caused by ovariectomy (P < 0.05). Whereas only the Est group showed evidence for reduced bone turnover compared with the Ovx group, MetR diet and/or endurance exercise demonstrated efficiencies in downregulating serum insulin, leptin, triglyceride, and thiobarbituric acid reactive substances (P < 0.05). Both the Exe and MetR groups showed higher femoral cortical and total volumetric bone mineral density (vBMD), but only the Exe and Est groups preserved cancellous bone volume and/or vBMD of distal femora (P < 0.05) compared with the Ovx group. After being normalized to body mass, femora of the MetR and MetR + Exe groups had relatively higher bending strength and dimension values followed by the Sham, Exe, and Est groups (P < 0.05). In conclusion, both MetR diet and endurance exercise improved cortical bone properties, but only endurance exercise preserved cancellous bone under estrogen deficiency.",
author = "Tsang-Hai Huang and Su, {I. Hsiu} and Lewis, {Jack L.} and Ming-Shi Chang and Hsu, {Ar Tyan} and Perrone, {Carmen E.} and Ables, {Gene P.}",
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Effects of methionine restriction and endurance exercise on bones of ovariectomized rats : A study of histomorphometry, densitometry, and biomechanical properties. / Huang, Tsang-Hai; Su, I. Hsiu; Lewis, Jack L.; Chang, Ming-Shi; Hsu, Ar Tyan; Perrone, Carmen E.; Ables, Gene P.

In: Journal of Applied Physiology, Vol. 119, No. 5, 01.09.2015, p. 517-526.

Research output: Contribution to journalArticle

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AU - Ables, Gene P.

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